Dataset: siRNA profiling of human cancer cell line MDA-MB-231 knocked down for CD146 mRNA
Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion...
Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion molecules play a pivotal role in this phenomenon by regulating cell-cell and cell-matrix interactions. CD146 (MCAM) is associated with advanced tumor stage in melanoma, prostate and ovarian cancers. For this study, the MDA-MB-231 cell line was used as a prototypic mesenchymal and invasive cell line, spontaneously expressing high levels of CD146. Using whole-genome DNA microarrays, we investigated genes for which expression was modified by CD146 down-regulation, obtained by siRNA or shRNA technology Experiment Overall Design: Expression profiles of two cell lines transfected by two differents ShRNA directed against CD146 were compared with expression profiles of 3 control cell lines (i.e. native cell line, empty plasmid and shRNA directed against GFP)
- Species:
- human
- Samples:
- 5
- Source:
- E-GEOD-11951
- Updated:
- Dec.12, 2014
- Registered:
- Sep.03, 2014
Sample |
---|
GSE11951GSM302367 |
GSE11951GSM302372 |
GSE11951GSM302373 |
GSE11951GSM302375 |
GSE11951GSM302377 |