ArrayExpress Uploader0human2906arrayexpress_sid4Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion...E-GEOD-11951/profile/8773/arrayexpressuploader05cancercellgenomelinemelanomaprostateDec.12, 2014Falsesirna-profiling-of-human-cancer-cell-line-mda-mb-2E-GEOD-11951_A-AFFY-44siRNA profiling of human cancer cell line MDA-MB-231 knocked down for CD146 mRNASep.03, 2014Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion molecules play a pivotal role in this phenomenon by regulating cell-cell and cell-matrix interactions. CD146 (MCAM) is associated with advanced tumor stage in melanoma, prostate and ovarian cancers. For this study, the MDA-MB-231 cell line was used as a prototypic mesenchymal and invasive cell line, spontaneously expressing high levels of CD146. Using whole-genome DNA microarrays, we investigated genes for which expression was modified by CD146 down-regulation, obtained by siRNA or shRNA technology Experiment Overall Design: Expression profiles of two cell lines transfected by two differents ShRNA directed against CD146 were compared with expression profiles of 3 control cell lines (i.e. native cell line, empty plasmid and shRNA directed against GFP)http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11951http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11951/samples/