{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSE11951GSM302367": {}}, {"GSE11951GSM302372": {}}, {"GSE11951GSM302373": {}}, {"GSE11951GSM302375": {}}, {"GSE11951GSM302377": {}}], "id": 2906, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion...", "geo_gse_id": "E-GEOD-11951", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 5, "tags": ["cancer", "cell", "genome", "line", "melanoma", "prostate"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "sirna-profiling-of-human-cancer-cell-line-mda-mb-2", "geo_id_plat": "E-GEOD-11951_A-AFFY-44", "name": "siRNA profiling of human cancer cell line MDA-MB-231 knocked down for CD146 mRNA", "created": "Sep.03, 2014", "summary": "Metastasis is a complex process involving loss of adhesion, migration, invasion and proliferation of cancer cells. Cell adhesion molecules play a pivotal role in this phenomenon by regulating cell-cell and cell-matrix interactions. CD146 (MCAM) is associated with advanced tumor stage in melanoma, prostate and ovarian cancers. For this study, the MDA-MB-231 cell line was used as a prototypic mesenchymal and invasive cell line, spontaneously expressing high levels of CD146. Using whole-genome DNA microarrays, we investigated genes for which expression was modified by CD146 down-regulation, obtained by siRNA or shRNA technology Experiment Overall Design: Expression profiles of two cell lines transfected by two differents ShRNA directed against CD146 were compared with expression profiles of 3 control cell lines (i.e. native cell line, empty plasmid and shRNA directed against GFP)", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11951", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-11951/samples/"}