BioGPS
  • Home
  • Help
  • Plugins
  • Datasets
  • Sign Up
  • Login
Examples: Gene Symbol(s), Gene Ontology, Splicing plugins, Melanoma datasets
advanced
Home › Plugin Library › PrimerBank

Plugin: PrimerBank

1 rating | 67 users | 81 layouts

PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time...

Registered by Andrew Su
Add to Layout
View your gene of interest

PrimerBank is a public resource for PCR primers. These primers are designed for gene expression detection or quantification (real-time PCR). PrimerBank contains over 306,800 primers covering most known human and mouse genes. There are several ways to search for primers: GenBank Accession, NCBI protein accession, NCBI Gene ID, Gene SymbolNew!, PrimerBank ID or Keyword (gene description) or you can blast your gene sequence against the primerbank Sequence DBNew!. The primer design algorithm has been extensively tested by real-time PCR experiments for PCR specificity and efficiency. We have tested 26,855 primer pairs that correspond to 27,681 mouse genes by Real Time PCR followed by agarose gel electrophoresis and sequencing of the PCR products. The design success rate is 82.6% (22,187 successful primer pairs) based on agarose gel electrophoresis. All experimental validation data for mouse primers are available from PrimerBank. In order to view, please follow the appropriate links seen on the primer information page. http://pga.mgh.harvard.edu/primerbank/

Species:
human, mouse

URL Template:
http://plugins.biogps.org/cgi-bin/primerbank.cgi?id={{EntrezGene}}

Updated:
Aug. 26, 2011

Registered:
Sept. 3, 2008


Please Login or Sign Up to post comments.

Tags

  • design
  • genetics
  • primer
  • primerbank
  • refseq

Often Used With

  • Gene expression/activity chart
  • Gene Identifiers
  • Gene Wiki
  • STRING - Proteins and their Interactions
  • Gene Expression Atlas (ArrayExpress Atlas)

Other Formats

JSON    XML
  • About
  • Blog
  • Help
  • FAQ
  • Downloads
  • API
  • iPhone App
  • Email updates
© 2023 The Scripps Research Institute. All rights reserved. (ver 94eefe6 )
  • Terms of Use