Dataset: Transcription profiling by array of human mammary epithelial cells grown in 2D monolayers with or without EGF and insulin, or 3D Matrigel culture, after treatment with 4-hydroxytamoxifen
MCF10A MycER cells were grown either on 2D monolayer culture in the presence of complete growth medium, in the absence of EGF and insulin...
MCF10A MycER cells were grown either on 2D monolayer culture in the presence of complete growth medium, in the absence of EGF and insulin or in 3D Matrigel culture for 20 days to form acinar structures. MycER was activated with 4-hydroxytamoxifen (4-OHT) in all samples for 2 or 48 h. Control cells were treated with ethanol. The aim of the study was to explore the effect of different growth conditions on gene expression changes in response to c-Myc activation.
- Species:
- human
- Samples:
- 9
- Source:
- E-MEXP-765
- Updated:
- Dec.12, 2014
- Registered:
- Sep.22, 2014
Factors:
(via ArrayExpress)
| Sample | media | dose | time | compound |
|---|---|---|---|---|
| 2D media without EGF and insulin 2h | 2D monolayer without EGF and insulin | 100 | 2 | 4-hydroxytamoxifen |
| 2D media without EGF and insulin 48h | 2D monolayer without EGF and insulin | 100 | 48 | 4-hydroxytamoxifen |
| 2D media without EGF and insulin control | 2D monolayer without EGF and insulin | 48 | none | |
| 2D normal media 2h | 2D monolayer with EGF and insulin | 100 | 2 | 4-hydroxytamoxifen |
| 2D normal media 48h | 2D monolayer with EGF and insulin | 100 | 48 | 4-hydroxytamoxifen |
| 2D normal media control | 2D monolayer with EGF and insulin | 48 | none | |
| 3D normal media 2h | 3D Matrigel culture | 100 | 2 | 4-hydroxytamoxifen |
| 3D normal media 48h | 3D Matrigel culture | 100 | 48 | 4-hydroxytamoxifen |
| 3D normal media control | 3D Matrigel culture | 48 | none |