Dataset: Transcription profiling of human embryonic kidney cells expressing apoptosis inducing proteins, time series
Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were...
Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were harvested and RNA extracted from the cells after over-expression of these 3 proteins at 12, 24 and 48 hours. A positive apoptosis inducer staurosporine and a negative control transfection reagent only were also included at each time point. Each sample had an experimental replicate.
- Species:
- human
- Samples:
- 30
- Source:
- E-MEXP-421
- PubMed:
- 15653641
- Updated:
- Dec.12, 2014
- Registered:
- Sep.22, 2014
Factors:
(via ArrayExpress)
| Sample | time | Genotype | compound |
|---|---|---|---|
| Aco1 12h a | 12 | ACO1 overexpression | |
| Aco1 12h a | 12 | ACO1 overexpression | |
| Aco1 24 h a | 24 | ACO1 overexpression | |
| Aco1 24 h a | 24 | ACO1 overexpression | |
| Aco1 48 h a | 48 | ACO1 overexpression | |
| Aco1 48 h a | 48 | ACO1 overexpression | |
| Mock transfection 12 h a | 12 | wild_type | |
| Mock transfection 12 h a | 12 | wild_type | |
| Mock transfection 24h a | 24 | wild_type | |
| Mock transfection 24h a | 24 | wild_type | |
| Mock transfection 48 h a | 48 | wild_type | |
| Mock transfection 48 h a | 48 | wild_type | |
| STK3 12 h a | 12 | STK3 overexpression | |
| STK3 12 h a | 12 | STK3 overexpression | |
| STK3 24 h a | 24 | STK3 overexpression | |
| STK3 24 h a | 24 | STK3 overexpression | |
| STK3 48 h a | 48 | STK3 overexpression | |
| STK3 48 h a | 48 | STK3 overexpression | |
| STS 12 h a | 12 | wild_type | staurosporine |
| STS 12 h a | 12 | wild_type | staurosporine |
| STS 24 h a | 24 | wild_type | staurosporine |
| STS 24 h a | 24 | wild_type | staurosporine |
| STS 48 h a | 48 | wild_type | staurosporine |
| STS 48 h a | 48 | wild_type | staurosporine |
| XBP1 12 h a | 12 | XBP1 overexpression | |
| XBP1 12 h a | 12 | XBP1 overexpression | |
| XBP1 24 h a | 24 | XBP1 overexpression | |
| XBP1 24 h a | 24 | XBP1 overexpression | |
| XBP1 48 h a | 48 | XBP1 overexpression | |
| XBP1 48 h a | 48 | XBP1 overexpression |