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Home › Dataset Library › Transcription profiling of human embryonic kidney cells expressing apoptosis inducing proteins, time series

Dataset: Transcription profiling of human embryonic kidney cells expressing apoptosis inducing proteins, time series

Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were...

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Three apoptosis inducing proteins, ACO1, STK3 and XBP1 were discovered in a previous reverse transfection screen. HEK293T cells were harvested and RNA extracted from the cells after over-expression of these 3 proteins at 12, 24 and 48 hours. A positive apoptosis inducer staurosporine and a negative control transfection reagent only were also included at each time point. Each sample had an experimental replicate.

Species:
human

Samples:
30

Source:
E-MEXP-421

PubMed:
15653641

Updated:
Dec.12, 2014

Registered:
Sep.22, 2014


Factors: (via ArrayExpress)
Sample time Genotype compound
Aco1 12h a 12 ACO1 overexpression
Aco1 12h a 12 ACO1 overexpression
Aco1 24 h a 24 ACO1 overexpression
Aco1 24 h a 24 ACO1 overexpression
Aco1 48 h a 48 ACO1 overexpression
Aco1 48 h a 48 ACO1 overexpression
Mock transfection 12 h a 12 wild_type
Mock transfection 12 h a 12 wild_type
Mock transfection 24h a 24 wild_type
Mock transfection 24h a 24 wild_type
Mock transfection 48 h a 48 wild_type
Mock transfection 48 h a 48 wild_type
STK3 12 h a 12 STK3 overexpression
STK3 12 h a 12 STK3 overexpression
STK3 24 h a 24 STK3 overexpression
STK3 24 h a 24 STK3 overexpression
STK3 48 h a 48 STK3 overexpression
STK3 48 h a 48 STK3 overexpression
STS 12 h a 12 wild_type staurosporine
STS 12 h a 12 wild_type staurosporine
STS 24 h a 24 wild_type staurosporine
STS 24 h a 24 wild_type staurosporine
STS 48 h a 48 wild_type staurosporine
STS 48 h a 48 wild_type staurosporine
XBP1 12 h a 12 XBP1 overexpression
XBP1 12 h a 12 XBP1 overexpression
XBP1 24 h a 24 XBP1 overexpression
XBP1 24 h a 24 XBP1 overexpression
XBP1 48 h a 48 XBP1 overexpression
XBP1 48 h a 48 XBP1 overexpression

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