Dataset: Transcription profiling by array of human akt-activated endothelial cells and mesenchymal progenitors to compare the cytokines and membrane factors that are differentially expressed

The goal of our experiment is to compare the cytokines and membrane factors that are differentially expressed between akt-activated endothelial cells and mesenchymal progenitors. Those two cell type have previously been described as potential niche for HSC expansion in vivo and in vitro. We isolated placental mesenchymal progenitors as previously described (Raynaud CM. et al. 2012 stem cell international). HUVECs cells were also isolated from cord and transfected with E4ORF1 as previously described (Seandel M. et al. 2008 PNAS). Both cell type were grown separately 3 days in X-Vivo media complemented with TPO, Flt3 and SCF (HSc amplification media). Pl-MPs and E4+ECs RNA was isolated using Trizol reagent followed by additional purification using RNAeasy extraction kit from Qiagen (74106, QIAGEN) with RNA yields that produces satisfactory microarray data. Two quality control measures were carried out: (1) A spectrophotometric analysis (2) A size fractionation procedure using a microfluidics instrument (Agilent Technologies). 200 ng of total RNA were analyzed on Affymetrix GeneChip Human Genome U133 Plus 2.0 Array. Data were analyzed using Parteck Software (V6.09.1110-6; Affimetrix).

Species:

human

Samples:

6

Source:

E-MEXP-3725

Updated:

Dec.12, 2014

Registered:

Jul.12, 2014