Dataset: Transcription profiling by array of myeloid progenitor cells

5000 Mac-1+, c-Kit+ myeloid progenitor cells from 3 mice homozygous for the p42-specific knockout Cebpa mutation and independently transplanted with leukemic bone marrow, or from 3 control mice heterozygous for Cebpa mutation, were isolated by FACS. Total RNA was extracted and fluorescence labeled for hybridization to MOE430.2 gene expression arrays. cRNA synthesis entailed 2 rounds of linear amplification using the T7 cRNA protocol provided by the manufacturer. Hybridization and washing was performed according to Affymetrix GeneChip expression analysis technical protocols. Microarray data were analyzed in the R statistical computing framework using the Bioconductor suite. Hybridization quality was assessed with the Bioconductor package arrayQualityMetrics and the raw fluorescence data were processed using the GCRMA method.

Species:

mouse

Samples:

6

Source:

E-MEXP-1444

PubMed:

18394553

Updated:

Dec.12, 2014

Registered:

Nov.21, 2014