Dataset: Transcription profiling of cerebellum cells from wild type mice or mice overexpressing TATA-binding protein (TBP) or a mutant version of TBP

[u'Transcription profiles were obtained for 2-month old mice. Three lines (WT, TBP-13Q12, and TBP105Q) were used in these experiments. ', {u'br': None}, {u'br': None}, u' Wild-type and 13Q mice are normal. The 13Q mice overexpress the normal protein on the RNA but not protein level and do not show a phenotype. The 105Q mice express a mutant version of the protein TBP and faithfully model the disease SCA17 (spinocerebellar ataxia-17, huntington disease-like 4, HDL4). ', {u'br': None}, {u'br': None}, u' The constructs containing mixed CAG/CAA trinucleotide repeats (and encoding polyglutamine tracts) of variable length were made using a previously described method (Michalik A et al., Biotechniques, 2001). Briefly, synthetic CAG/CAA oligonucleotides were subcloned into a cDNA construct of the normal mouse (13 CAG/CAA) TBP gene. Because of the mixed nature of the repeat, its length is stable in mitotic and meiotic transmission.']

Species:

mouse

Samples:

9

Source:

E-MEXP-1314

PubMed:

17994014

Updated:

Dec.12, 2014

Registered:

Nov.21, 2014