Dataset: Transcription profiling of mouse CD4+ T cells expressing Foxp3 FACS isolated from hemizygouse males to investigate development of regulatory t cell precursors in the absence of a functional foxp3 protein

To analyze gene expression in in regulatory T cell precursors that develop in the absence of a functional Foxp3 protein as compared to that of normal regulatory T cells Experiment Overall Design: Murine wild-type and mutant (delta) Foxp3 alleles were tagged with EGFP reporters. CD4 positive T cells expressing the respective tagged Foxp3 allele (Foxp3/EGFP and delta Foxp3/EGFP) were isolated from hemizygous male mice by cell sorting using FACS. CD4 positive cells that did not express the EGFP tag were a isolated. Total RNA was prepared and used in the array studies.

Species:

mouse

Samples:

8

Source:

E-GEOD-6875

Updated:

Dec.12, 2014

Registered:

Nov.13, 2014