Dataset: Dilution and amplification control study
Mouse adult female brain’s cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80°C. Subsequently,...
Mouse adult female brain’s cortex (C57BL/6, Charles River Laboratories, Inc.) was isolated and stored immediately at -80°C. Subsequently, the mRNA (15µg) was isolated using TRIzol Reagent and MicroFastTrack 2.0 Kit (Invitrogen). A Sample of 5µg was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently aRNA amplified for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays. A Sample of 5µg of mRNA was assessed on Affymetrix Mouse 430.2 array. Aliquots from the leftovers of the same cortical mRNA were diluted to single-cell RNA levels (0.1, 1, and 10 pg) and independently amplified, as described above, for a total of 2 and 4 rounds and assessed on Affymetrix Mouse 430.2 arrays.
- Species:
- mouse
- Samples:
- 17
- Source:
- E-GEOD-61081
- Updated:
- Dec.12, 2014
- Registered:
- Nov.12, 2014
| Sample |
|---|
| GSM1496307 1 |
| GSM1496308 1 |
| GSM1496309 1 |
| GSM1496310 1 |
| GSM1496311 1 |
| GSM1496312 1 |
| GSM1496313 1 |
| GSM1496314 1 |
| GSM1496315 1 |
| GSM1496316 1 |
| GSM1496317 1 |
| GSM1496318 1 |
| GSM1496319 1 |
| GSM1496320 1 |
| GSM1496321 1 |
| GSM1496322 1 |
| GSM1496323 1 |