Dataset: Transcriptional analysis of iPSCs during cellular reprogramming using RNA FACS
Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2,...
Mouse induced pluripotent stem cells (iPSCs) were derived from embryonic fibroblasts by overexpressing the Yamanaka factors Oct4, Sox2, Klf4 and c-Myc for 32 consecutive days. iPSCs were isolated by RNA FACS (endogenous Sox2) from the heterogeneous reprogramming culture and transcriptionally compared with (1) iPSCs stabilized in 2i medium (iPS-2i) and (2) iPSCs stabilized in co-culture with mouse embryonic fibroblasts (iPS-MEF) n = 2 replicates for each sample/condition
- Species:
- mouse
- Samples:
- 10
- Source:
- E-GEOD-55671
- Updated:
- Dec.12, 2014
- Registered:
- Nov.24, 2014
Factors:
(via ArrayExpress)
| Sample | SAMPLE TYPE |
|---|---|
| GSM1341447 | grown in 2i/serum |
| GSM1341447 | grown in 2i/serum |
| GSM1341449 | co-cultured with MEFs |
| GSM1341449 | co-cultured with MEFs |
| GSM134145 | secondary MEFs following 32 days of Yamanaka factor expression |
| GSM134145 | secondary MEFs following 32 days of Yamanaka factor expression |
| GSM1341453 | secondary MEFs following 32 days of Yamanaka factor expression, sox2 postive fraction |
| GSM1341453 | secondary MEFs following 32 days of Yamanaka factor expression, sox2 postive fraction |
| GSM1341455 | secondary MEFs following 32 days of Yamanaka factor expression, sox2 negative fraction |
| GSM1341455 | secondary MEFs following 32 days of Yamanaka factor expression, sox2 negative fraction |