Dataset: Specific extracellular matrix remodeling signature of colon hepatic metastases

To identify genes implicated in metastatic colonization of the liver in colorectal cancer, we collected pairs of primary tumors and...

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To identify genes implicated in metastatic colonization of the liver in colorectal cancer, we collected pairs of primary tumors and hepatic metastases before chemotherapy in 13 patients. We compared mRNA expression in the pairs of patients to identify genes deregulated during metastatic evolution. We then validated the identified genes using data obtained by different groups. The 33-gene signature was able to classify 87% of hepatic metastases, 98% of primary tumors, 97% of normal colon mucosa, and 95% of normal liver tissues in six datasets obtained using five different microarray platforms. The identified genes are specific to colon cancer and hepatic metastases since other metastatic locations and hepatic metastases originating from breast cancer were not classified by the signature. Gene Ontology term analysis showed that 50% of the genes are implicated in extracellular matrix remodeling, and more precisely in cell adhesion, extracellular matrix organization and angiogenesis. Because of the high efficiency of the signature to classify colon hepatic metastases, the identified genes represent promising targets to develop new therapies that will specifically affect hepatic metastasis microenvironment. 57 samples of patients with stage IV colorectal cancer. We compared using Affymetrix chips gene expression profiles between primary tumors and hepatic metastases

Species:: human
Samples:: 57
Source:: E-GEOD-49355
Updated:: Jan.17, 2015
Registered:: Jan.17, 2015

Factors: (via ArrayExpress)

Sample	PATIENT NUMBER	TUMOR LOCATION	ORGANISM PART	PT	AGE	SEX	DIFFERENTIATION	PN	WHO PERFORMANCE STATUS
GSM1197996 1	1	Left colon	Primary Tumor	pT3	51	female	Well	pN2	1
GSM1197997 1	1	Left colon	Normal Colon	pT3	51	female	Well	pN2	1
GSM1197998 1	1	Left colon	Liver Metastasis	pT3	51	female	Well	pN2	1
GSM1197999 1	7	Rectum-sigmoid junction	Liver Metastasis	pT3	53	male	not specified	pN0	not specified
GSM1198000 1	8	Left colon	Liver Metastasis	pT3	49	male	Moderate	pN2	0
GSM1198001 1	21	Left colon	Primary Tumor	pT3	68	female	Well	pN2	1
GSM1198002 1	21	Left colon	Normal Colon	pT3	68	female	Well	pN2	1
GSM1198003 1	30	Left colon	Normal Colon	pT4	72	male	not specified	pN0	0
GSM1198004 1	42	Left colon	Liver Metastasis	pT4	50	female	Well	pN1	1
GSM1198005 1	42	Left colon	Normal Colon	pT4	50	female	Well	pN1	1
GSM1198006 1	42	Left colon	Primary Tumor	pT4	50	female	Well	pN1	1
GSM1198007 1	43	Left colon	Primary Tumor	pT3	71	male	Well	pN2	1
GSM1198008 1	50	rectum	Liver Metastasis	pT3	68	male	Well	pN2	0
GSM1198009 1	51	Left colon	Liver Metastasis	pT4	65	male	Moderate	pN0	0
GSM1198010 1	51	Left colon	Primary Tumor	pT4	65	male	Moderate	pN0	0
GSM1198011 1	56	Left colon	Liver Metastasis	pT3	60	male	Well	pN2	0
GSM1198012 1	56	Left colon	Primary Tumor	pT3	60	male	Well	pN2	0
GSM1198013 1	56	Left colon	Normal Colon	pT3	60	male	Well	pN2	0
GSM1198014 1	64	Left colon	Primary Tumor	pT3	64	male	Moderate	pN2	0
GSM1198015 1	64	Left colon	Normal Colon	pT3	64	male	Moderate	pN2	0
GSM1198016 1	77	Left colon	Normal Colon	pT3	62	male	Well	pN2	1
GSM1198017 1	77	Left colon	Liver Metastasis	pT3	62	male	Well	pN2	1
GSM1198018 1	77	Left colon	Primary Tumor	pT3	62	male	Well	pN2	1
GSM1198019 1	93	Rectum-sigmoid junction	Liver Metastasis	pT4	50	male	Poor	pN2	1
GSM1198020 1	93	Rectum-sigmoid junction	Primary Tumor	pT4	50	male	Poor	pN2	1
GSM1198021 1	94	transverse colon	Normal Colon	pT4	64	female	Moderate	pN2	0
GSM1198022 1	94	transverse colon	Liver Metastasis	pT4	64	female	Moderate	pN2	0
GSM1198023 1	103	rectum	Liver Metastasis	pT4	54	male	Well	pN1	0
GSM1198024 1	108	Right colon	Normal Colon	pT3	45	female	Moderate	pN2	1
GSM1198025 1	108	Right colon	Liver Metastasis	pT3	45	female	Moderate	pN2	1
GSM1198026 1	108	Right colon	Primary Tumor	pT3	45	female	Moderate	pN2	1
GSM1198027 1	109	Left colon	Normal Colon	pT3	69	female	not specified	pN2	0
GSM1198028 1	109	Left colon	Primary Tumor	pT3	69	female	not specified	pN2	0
GSM1198029 1	123	Left colon	Primary Tumor	pT3	56	female	Poor	pN2	0
GSM1198030 1	141	Left colon	Primary Tumor	pT3	57	male	Well	pN0	0
GSM1198031 1	141	Left colon	Liver Metastasis	pT3	57	male	Well	pN0	0
GSM1198032 1	141	Left colon	Normal Colon	pT3	57	male	Well	pN0	0
GSM1198033 1	174	rectum	Liver Metastasis	not specified	76	male	not specified	not specified	0
GSM1198034 1	178	Left colon	Normal Colon	pT3	54	male	Well	pN0	1
GSM1198035 1	178	Left colon	Primary Tumor	pT3	54	male	Well	pN0	1
GSM1198036 1	185	Left colon	Liver Metastasis	pT3	60	female	Well	pN1	0
GSM1198037 1	185	Left colon	Primary Tumor	pT3	60	female	Well	pN1	0
GSM1198038 1	185	Left colon	Normal Colon	pT3	60	female	Well	pN1	0
GSM1198039 1	202	Left colon	Liver Metastasis	pT3	53	female	Moderate	pN1	1
GSM1198040 1	202	Left colon	Normal Colon	pT3	53	female	Moderate	pN1	1
GSM1198041 1	202	Left colon	Primary Tumor	pT3	53	female	Moderate	pN1	1
GSM1198042 1	209	Left colon	Liver Metastasis	pT3	52	male	Moderate	pN0	1
GSM1198043 1	209	Left colon	Primary Tumor	pT3	52	male	Moderate	pN0	1
GSM1198044 1	209	Left colon	Normal Colon	pT3	52	male	Moderate	pN0	1
GSM1198045 1	211	Left colon	Normal Colon	pT4	61	male	Poor	pN2	1
GSM1198046 1	211	Left colon	Primary Tumor	pT4	61	male	Poor	pN2	1
GSM1198047 1	234	caecum	Normal Colon	pT3	57	female	Moderate	pN1	not specified
GSM1198048 1	238	Left colon	Primary Tumor	pT3	70	male	Moderate	pN2	0
GSM1198049 1	238	Left colon	Liver Metastasis	pT3	70	male	Moderate	pN2	0
GSM1198050 1	238	Left colon	Normal Colon	pT3	70	male	Moderate	pN2	0
GSM1198051 1	261	transverse colon	Primary Tumor	pT3	59	female	Moderate	pN1	1
GSM1198052 1	261	transverse colon	Liver Metastasis	pT3	59	female	Moderate	pN1	1