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Home › Dataset Library › Expression data from T lymphocytes derived from T-iPS and peripheral blood

Dataset: Expression data from T lymphocytes derived from T-iPS and peripheral blood

T lymphocytes can be generated from T-cell-derived induced pluripotent stem cells (T-iPS). We used microarrays to better elucidate their...

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T lymphocytes can be generated from T-cell-derived induced pluripotent stem cells (T-iPS). We used microarrays to better elucidate their phenotype and compare their gene expression profile to that of known lymhoid subsets from peripheral blood. Total mRNA was extracted from T-iPS-derived T cells at day 30-35 of differentiation and from sorted peripheral blood lymphoid subpopulations. Total RNA was amplified and labelled following the standard Affymetrix protocol (Affymetrix, Santa Clara, CA, USA). The labeled complementary RNA was then fragmented and hybridized to Affymetrix GeneChip arrays HG-U133 plus 2.0.

Species:
human

Samples:
11

Source:
E-GEOD-47920

Updated:
Dec.12, 2014

Registered:
Jul.12, 2014


Factors: (via ArrayExpress)
Sample CELL TYPE
GSM1162426 CD3+TCRγδ+ sorted T cells from peripheral blood
GSM1162426 CD3+TCRγδ+ sorted T cells from peripheral blood
GSM1162424 CD3+CD56+ sorted T cells from peripheral blood
GSM1162424 CD3+CD56+ sorted T cells from peripheral blood
GSM1162422 CD3+CD8+ sorted T cells from peripheral blood
GSM1162422 CD3+CD8+ sorted T cells from peripheral blood
GSM1162420 CD3+CD4+ sorted T cells from peripheral blood
GSM1162420 CD3+CD4+ sorted T cells from peripheral blood
GSM1162418 T-iPS-derived T cells at day 30 of differentiation
GSM1162417 T-iPS-derived T cells at day 35 of differentiation
GSM1162416 T-iPS-derived T cells at day 33 of differentiation

Tags

  • cell
  • peripheral

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