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Home › Dataset Library › Testing rescue of AR signaling by ectopic expression of mutant AR allele in the presence of MDV-3100

Dataset: Testing rescue of AR signaling by ectopic expression of mutant AR allele in the presence of MDV-3100

Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection...

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Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection This experiment is designed to see if expressing the F876L/T877A mutant AR can rescue AR signaling in the presence of MDV3100 Engineered lines were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 10uM MDV3100 for 24h

Species:
human

Samples:
16

Source:
E-GEOD-44924

Updated:
Dec.12, 2014

Registered:
Jul.11, 2014


Factors: (via ArrayExpress)
Sample MDV SENSITIVITY GENOTYPE COMPOUND
GSM1093972 partially insensitive W741C/T877A Mut overexpression 10uM MDV3100
GSM1093972 partially insensitive W741C/T877A Mut overexpression 10uM MDV3100
GSM1093970 partially insensitive F876L/T877A Mut overexpression 10uM MDV3100
GSM1093970 partially insensitive F876L/T877A Mut overexpression 10uM MDV3100
GSM1093968 sensitive T877A WT overexpression 10uM MDV3100
GSM1093968 sensitive T877A WT overexpression 10uM MDV3100
GSM1093966 sensitive Vec control 10uM MDV3100
GSM1093966 sensitive Vec control 10uM MDV3100
GSM1093964 partially insensitive W741C/T877A Mut overexpression 0.1% DMSO
GSM1093964 partially insensitive W741C/T877A Mut overexpression 0.1% DMSO
GSM1093962 partially insensitive F876L/T877A Mut overexpression 0.1% DMSO
GSM1093962 partially insensitive F876L/T877A Mut overexpression 0.1% DMSO
GSM1093960 sensitive T877A WT overexpression 0.1% DMSO
GSM1093960 sensitive T877A WT overexpression 0.1% DMSO
GSM1093958 sensitive Vec control 0.1% DMSO
GSM1093958 sensitive Vec control 0.1% DMSO

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