Dataset: Testing rescue of AR signaling by ectopic expression of mutant AR allele in the presence of MDV-3100
Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection...
Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection This experiment is designed to see if expressing the F876L/T877A mutant AR can rescue AR signaling in the presence of MDV3100 Engineered lines were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 10uM MDV3100 for 24h
- Species:
- human
- Samples:
- 16
- Source:
- E-GEOD-44924
- Updated:
- Dec.12, 2014
- Registered:
- Jul.11, 2014
Factors:
(via ArrayExpress)
Sample | MDV SENSITIVITY | GENOTYPE | COMPOUND |
---|---|---|---|
GSM1093972 | partially insensitive | W741C/T877A Mut overexpression | 10uM MDV3100 |
GSM1093972 | partially insensitive | W741C/T877A Mut overexpression | 10uM MDV3100 |
GSM1093970 | partially insensitive | F876L/T877A Mut overexpression | 10uM MDV3100 |
GSM1093970 | partially insensitive | F876L/T877A Mut overexpression | 10uM MDV3100 |
GSM1093968 | sensitive | T877A WT overexpression | 10uM MDV3100 |
GSM1093968 | sensitive | T877A WT overexpression | 10uM MDV3100 |
GSM1093966 | sensitive | Vec control | 10uM MDV3100 |
GSM1093966 | sensitive | Vec control | 10uM MDV3100 |
GSM1093964 | partially insensitive | W741C/T877A Mut overexpression | 0.1% DMSO |
GSM1093964 | partially insensitive | W741C/T877A Mut overexpression | 0.1% DMSO |
GSM1093962 | partially insensitive | F876L/T877A Mut overexpression | 0.1% DMSO |
GSM1093962 | partially insensitive | F876L/T877A Mut overexpression | 0.1% DMSO |
GSM1093960 | sensitive | T877A WT overexpression | 0.1% DMSO |
GSM1093960 | sensitive | T877A WT overexpression | 0.1% DMSO |
GSM1093958 | sensitive | Vec control | 0.1% DMSO |
GSM1093958 | sensitive | Vec control | 0.1% DMSO |