BioGPS
  • Home
  • Help
  • Plugins
  • Datasets
  • Sign Up
  • Login
Examples: Gene Symbol(s), Gene Ontology, Splicing plugins, Melanoma datasets
advanced
Home › Dataset Library › Preeclampsia: the in vivo milieu leads to cytotrophoblast dysregulation

Dataset: Preeclampsia: the in vivo milieu leads to cytotrophoblast dysregulation

During human pregnancy, placental cytotrophoblasts invade the uterus and its blood vessels, anchoring the progeny and rerouting maternal...

Registered by ArrayExpress Uploader
View Dataset

During human pregnancy, placental cytotrophoblasts invade the uterus and its blood vessels, anchoring the progeny and rerouting maternal blood to the embryo/fetus. In preeclampsia, cytotrophoblast invasion is restricted and blood flow to the placenta is reduced. The causes of restricted cytotrophoblast invasion are unknown. Here, preeclampsia and control cytotrophoblasts were cultured for 48 h to allow differentiation/invasion. In various severe forms of preeclampsia ± intrauterine growth restriction, global transcriptional profiling revealed common aberrations in cytotrophoblast gene expression that resolved with culture. Villous cytotrophoblasts were isolated from preeclampsia placentas (PRE, n=5) and placentas of preterm labor patients without signs of infection (PTL, n=5), which served as gestation-matched controls. To better understand the CTB phenotype in the context of PE variants, we included patients with the most clinically significant forms of this condition that necessitated preterm delivery: women with severe PE ± intrauterine growth restrictions, PE with superimposed hypertension and HELLP syndrome (hemolysis, elevated liver enzymes; low platelet count). RNA was purified immediately after the cells were isolated (0 h) and after 12, 24 and 48 h in culture. The relative gene expression across the whole genome was profiled using the Affymetrix HG-U133Plus 2.0 GeneChip platform. Array quality was assesed using RMAExpress. One sample of preterm labor collected at 48h was omitted (39 arrays total). We used both LIMMA and maSigPro (R/Bioconductor) to determine differentially expressed genes.

Species:
human

Samples:
39

Source:
E-GEOD-40182

Updated:
Dec.12, 2014

Registered:
Sep.19, 2014


Factors: (via ArrayExpress)
Sample CULTURE TIME HRS PHENOTYPE
GSM987946 not specified preterm labor
GSM987946 not specified preterm labor
GSM987946 not specified preterm labor
GSM987946 not specified preterm labor
GSM987946 not specified preterm labor
GSM98795 12 preterm labor
GSM98795 12 preterm labor
GSM98795 12 preterm labor
GSM98795 12 preterm labor
GSM98795 12 preterm labor
GSM987956 24 preterm labor
GSM987956 24 preterm labor
GSM987956 24 preterm labor
GSM987956 24 preterm labor
GSM987956 24 preterm labor
GSM98796 48 preterm labor
GSM98796 48 preterm labor
GSM98796 48 preterm labor
GSM98796 48 preterm labor
GSM987965 not specified preeclampsia
GSM987965 not specified preeclampsia
GSM987965 not specified preeclampsia
GSM987965 not specified preeclampsia
GSM987965 not specified preeclampsia
GSM987970 12 preeclampsia
GSM987970 12 preeclampsia
GSM987970 12 preeclampsia
GSM987970 12 preeclampsia
GSM987970 12 preeclampsia
GSM987975 24 preeclampsia
GSM987975 24 preeclampsia
GSM987975 24 preeclampsia
GSM987975 24 preeclampsia
GSM987975 24 preeclampsia
GSM987980 48 preeclampsia
GSM987980 48 preeclampsia
GSM987980 48 preeclampsia
GSM987980 48 preeclampsia
GSM987980 48 preeclampsia

Tags

  • cytotrophoblast
  • embryo
  • fetus
  • genome
  • hellp syndrome
  • hypertension
  • liver
  • placenta
  • platelet
  • preeclampsia
  • syndrome
  • uterus

Other Formats

JSON    XML
  • About
  • Blog
  • Help
  • FAQ
  • Downloads
  • API
  • iPhone App
  • Email updates
© 2023 The Scripps Research Institute. All rights reserved. (ver 94eefe6 )
  • Terms of Use