ArrayExpress Uploader0humannot specifiedpreterm labornot specifiedpreterm labornot specifiedpreterm labornot specifiedpreterm labornot specifiedpreterm labor12preterm labor12preterm labor12preterm labor12preterm labor12preterm labor24preterm labor24preterm labor24preterm labor24preterm labor24preterm labor48preterm labor48preterm labor48preterm labor48preterm labornot specifiedpreeclampsianot specifiedpreeclampsianot specifiedpreeclampsianot specifiedpreeclampsianot specifiedpreeclampsia12preeclampsia12preeclampsia12preeclampsia12preeclampsia12preeclampsia24preeclampsia24preeclampsia24preeclampsia24preeclampsia24preeclampsia48preeclampsia48preeclampsia48preeclampsia48preeclampsia48preeclampsia4555arrayexpress_sid4During human pregnancy, placental cytotrophoblasts invade the uterus and its blood vessels, anchoring the progeny and rerouting maternal...E-GEOD-40182/profile/8773/arrayexpressuploader239cytotrophoblastembryofetusgenomehellp syndromehypertensionliverplacentaplateletpreeclampsiasyndromeuterusDec.12, 2014Falsepreeclampsia-the-in-vivo-milieu-leads-to-cytotrophE-GEOD-40182_A-AFFY-44Preeclampsia: the in vivo milieu leads to cytotrophoblast dysregulationSep.19, 2014During human pregnancy, placental cytotrophoblasts invade the uterus and its blood vessels, anchoring the progeny and rerouting maternal blood to the embryo/fetus. In preeclampsia, cytotrophoblast invasion is restricted and blood flow to the placenta is reduced. The causes of restricted cytotrophoblast invasion are unknown. Here, preeclampsia and control cytotrophoblasts were cultured for 48 h to allow differentiation/invasion. In various severe forms of preeclampsia ± intrauterine growth restriction, global transcriptional profiling revealed common aberrations in cytotrophoblast gene expression that resolved with culture. Villous cytotrophoblasts were isolated from preeclampsia placentas (PRE, n=5) and placentas of preterm labor patients without signs of infection (PTL, n=5), which served as gestation-matched controls. To better understand the CTB phenotype in the context of PE variants, we included patients with the most clinically significant forms of this condition that necessitated preterm delivery: women with severe PE ± intrauterine growth restrictions, PE with superimposed hypertension and HELLP syndrome (hemolysis, elevated liver enzymes; low platelet count). RNA was purified immediately after the cells were isolated (0 h) and after 12, 24 and 48 h in culture. The relative gene expression across the whole genome was profiled using the Affymetrix HG-U133Plus 2.0 GeneChip platform. Array quality was assesed using RMAExpress. One sample of preterm labor collected at 48h was omitted (39 arrays total). We used both LIMMA and maSigPro (R/Bioconductor) to determine differentially expressed genes.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40182http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-40182/samples/