Dataset: Expression data from Bone-Marrow Mesenchymal Stromal Cells (BM-MSC) from Follicular Lymphoma patient (FL) or Healthy Donor (HD).

Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of...

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Human bone marrow mesenchymal stromal cells (BM-MSC) could be committed toward a functional lymphoid-like stroma by a combination of TNFalpha (TNF) and Lymphotoxin alpha1/beta2 (LT) (Amé-Thomas et al Blood 2007). Bone marrow and lymph node stromal cells support FL malignant cell recruitment and growth in particular after comittment to a lymphoid-like differentiation in vitro. In addition, more than 70% of FL patients exhibit a bone marrow involvment at diagnosis. We delineate using Affymetrix U133+2.0 microarrays the gene expression profile of BM-MSC obtained from FL patients (FL-MSC) and age-matched healthy donors (HD-MSC) in order to identify a specific FL-MSC signature. In addition, we used Affymetrix microarrays to define the gene expression signature of lymphoid-like stromal cells obtained from HD-MSC by treatment with TNF/LT in vitro. This TNF/LT signature was then used to interpret the gene expression profile of FL-MSC. GEP was performed on 10 BM-MSC from FL patients and 6 from healthy donors, treated or not with TNF(10 ng/mL)/LT(100ng/mL)

Species:: human
Samples:: 28
Source:: E-GEOD-35331
Updated:: Dec.12, 2014
Registered:: Sep.19, 2014

Factors: (via ArrayExpress)

Sample	AGE (Y)	GROWTH PROTOCOL	PATHOLOGY	TREATMENT	GENDER
GSM866168	55	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866169	53	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866170	30	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM86617	68	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	female
GSM866169	53	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866173	78	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	female
GSM866174	68	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866175	55	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866176	76	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866177	53	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	female
GSM866178	73	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866179	61	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866180	47	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM86618	56	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866182	42	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	male
GSM866183	66	alphaMEM+10%FBS+FGF2(1ng/mL)	Follicular Lymphoma patient	Untreated	female
GSM866168	55	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866169	53	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866170	30	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM86617	68	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	female
GSM866169	53	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	male
GSM866173	78	alphaMEM+10%FBS+FGF2(1ng/mL)	Healthy donor	Untreated	female
GSM866190	55	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	male
GSM86619	53	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	male
GSM866192	30	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	male
GSM866193	68	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	female
GSM86619	53	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	male
GSM866195	78	RPMI1640+10%FBS	Healthy donor	Stimulated by TNF+LT for 3 days	female