Dataset: Trans-splicing in human embryonic stem cells

Trans-spliced isoform of NCRMS (tsNCRMS) tends to be specifically transcribed in hESCs/iPSCs and disruption of tsNCRMS expression can conspicuously affect the pluripotency maintenance of hESCs. We disrupt the expression of tsNCRMS by introducing tsNCRMS shTS2 that target the no-co-linear junction site in hESCs using lentiviral vectors and use shLuc as control.

Species:

human

Samples:

4

Source:

E-GEOD-32503

Updated:

Dec.12, 2014

Registered:

Jul.11, 2014