Dataset: Expression data from naive and memory CD8 T cells cultured in presence or absence of IL-4
Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies...
Effects of IL-4 on CD8 T cells functions are largely unknown. IL-4 induces survival and proliferation of CD8 T cells, but several studies suggest that IL-4 could also affect several functions of CD8 T cells such as cytotoxicity. Our team has shown that IL-4 repress the expression of Ccl5 in vitro. To define more precisely the impact of IL-4 on CD8 T cells, we performed a whole genome expression microarray analysis of naive and memory CD8 T cells cultured in presence or absence of IL-4. This approach allowed us to define the IL4-gene-expression signature on CD8 T cells. 18 samples were processed. Two populations of F5 naive CD8 T cells were FACS-sorted: samples from each population were incubated 20 hours with IL-7 in presence or absence of IL-4. Thus, a total of 6 “Naive” samples were processed. In addition, 4 populations of F5 TIM memory CD8 T cells were FACS-sorted: samples from 2 of these populations were incubated 20 hours in presence of IL-7 and/or IL-4, or in medium alone. Thus, 12 “Memory” samples were processed.
- Species:
- mouse
- Samples:
- 18
- Source:
- E-GEOD-32423
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
| Sample | TREATMENT | CELL TYPE |
|---|---|---|
| GSM802439 | not specified | Naïve CD8 memory cells |
| GSM802439 | not specified | Naïve CD8 memory cells |
| GSM80244 | not specified | Tim CD8 memory cells |
| GSM80244 | not specified | Tim CD8 memory cells |
| GSM80244 | not specified | Tim CD8 memory cells |
| GSM80244 | not specified | Tim CD8 memory cells |
| GSM802445 | incubated with IL7, for 20h in vitro | Naïve CD8 memory cells incubated with IL7, for 20h in vitro |
| GSM802445 | incubated with IL7, for 20h in vitro | Naïve CD8 memory cells incubated with IL7, for 20h in vitro |
| GSM802447 | incubated with IL4 and IL7, for 20h in vitro | Naïve CD8 memory cells incubated with IL4 and IL7, for 20h in vitro |
| GSM802447 | incubated with IL4 and IL7, for 20h in vitro | Naïve CD8 memory cells incubated with IL4 and IL7, for 20h in vitro |
| GSM802449 | incubated with IL4 and IL7, for 20h in vitro | Tim CD8 memory cells incubated with IL4 and IL7, for 20h in vitro |
| GSM802449 | incubated with IL4 and IL7, for 20h in vitro | Tim CD8 memory cells incubated with IL4 and IL7, for 20h in vitro |
| GSM80245 | incubated with IL4 for 20h in vitro | Tim CD8 memory cells incubated with IL4 for 20h in vitro |
| GSM80245 | incubated with IL4 for 20h in vitro | Tim CD8 memory cells incubated with IL4 for 20h in vitro |
| GSM802453 | incubated with IL7 for 20h in vitro | Tim CD8 memory cells incubated with IL7 for 20h in vitro |
| GSM802453 | incubated with IL7 for 20h in vitro | Tim CD8 memory cells incubated with IL7 for 20h in vitro |
| GSM802455 | incubated 20h in vitro | Tim CD8 memory cells incubated 20h in vitro |
| GSM802455 | incubated 20h in vitro | Tim CD8 memory cells incubated 20h in vitro |