Dataset: Genome-wide activity-dependent MeCP2 phosphorylation regulates nervous system development and function [cultured cortical neurons]
To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from...
To examine potential differences in activity-dependent gene expression, we analyzed mRNA expression in cultured neurons isolated from Wild-type vs MeCP2 S421A mice at 0 (unstimulated), 1 or 6 hours after membrane depolarization by exposure to high extracellular KCl (55mM) All mice were male littermates from one of three litters. We isolated RNA from dissociated cortical cultures (E16+7DIV) isolated from Wild-Type or MeCP2 S421A knock-in mice littermates. Cells were either left unstimulated or depolarized for 1 or 6 hours by addition of 55mM KCl to the media. mRNA expression was analyzed using the Affymetrix GeneChip Mouse Expression Set 430 2.0 microarray platform.
- Species:
- mouse
- Samples:
- 18
- Source:
- E-GEOD-31849
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
| Sample | LITTER | GENOTYPE/VARIATION | CONDITION |
|---|---|---|---|
| GSM789948 | A | WT | unstimulated |
| GSM789949 | A | MeCP2 S421A | unstimulated |
| GSM789950 | A | WT | 1 hr KCl depolarized |
| GSM78995 | A | MeCP2 S421A | 1 hr KCl depolarized |
| GSM789952 | A | WT | 6 hr KCl depolarized |
| GSM789953 | A | MeCP2 S421A | 6 hr KCl depolarized |
| GSM789954 | B | WT | unstimulated |
| GSM789955 | B | MeCP2 S421A | unstimulated |
| GSM789956 | B | WT | 1 hr KCl depolarized |
| GSM789957 | B | MeCP2 S421A | 1 hr KCl depolarized |
| GSM789958 | B | WT | 6 hr KCl depolarized |
| GSM789959 | B | MeCP2 S421A | 6 hr KCl depolarized |
| GSM789960 | C | WT | unstimulated |
| GSM78996 | C | MeCP2 S421A | unstimulated |
| GSM789962 | C | WT | 1 hr KCl depolarized |
| GSM789963 | C | MeCP2 S421A | 1 hr KCl depolarized |
| GSM789964 | C | WT | 6 hr KCl depolarized |
| GSM789965 | C | MeCP2 S421A | 6 hr KCl depolarized |