Dataset: Activation of SREBP in Alveolar Type II Cells Enhances Lipogenesis Causing Pulmonary Lipotoxicity
Background: Lung function is dependent upon the precise regulation of the synthesis, storage, and catabolism of tissue and alveolar...
Background: Lung function is dependent upon the precise regulation of the synthesis, storage, and catabolism of tissue and alveolar lipids. Results: Activation of SREBP (Sterol Response Element Binding Protein) induced lipogenesis in alveolar epithelial cells, causing neutral lipid accumulation, lung inflammation, and tissue remodeling. Conclusions: The accumulation of neutral lipids in type II epithelial cells and alveolar macrophages caused lung inflammation, consistent with findings in lipid storage disorders. Significance: Pulmonary lipotoxicity may contribute to the pathogenesis of lung dysfunction associated with diabetes, obesity, and other metabolic disorders. Genome-wide transcription profiling comparison between doxycycline-exposed SFTPC-rtTAWT/Tg/(tetO)7CMV-CreWT/Tg/Insig1flox/flox/Insig2-/- mice (i.e., Insig1/2∆/∆ ) and Insig1flox/flox/Insig2-/- . Three independent pooled RNA from isolated lung type 2 cells of each genotype were used.
- Species:
- mouse
- Samples:
- 6
- Source:
- E-GEOD-31797
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | STRAIN OR LINE |
---|---|
GSM788602 | Insig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |
GSM788602 | Insig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |
GSM788602 | Insig1 flox/flox /Insig2 -/- ( AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |
GSM78863 | Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |
GSM78863 | Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |
GSM78863 | Insig1 ∆/∆ /Insig2 -/- (AT2 cells obtained from 2 months old SpC-rTTA / Otet-CRE / Insig1 flox/flox / Insig2-/- mice treated with doxycycline from E6.5 to E12.5. Control animals lacked either SpC-rtTA or Otet-CRE) |