Dataset: Functional Plasticity of Regulatory T Cell Function
Regulatory T cells (Tregs) can suppress a wide variety of cell types, in diverse organ sites and inflammatory conditions. While Tregs...
Regulatory T cells (Tregs) can suppress a wide variety of cell types, in diverse organ sites and inflammatory conditions. While Tregs possess multiple suppressive mechanisms, the number required for maximal function is unclear. Furthermore, whether any inter-relationship orcross-regulatory mechanisms exist that areused to orchestrate and control their utilization is unknown. Here we assessed the functional capacity of Tregs lacking the ability to secrete both interleukin-10 (IL-10) and IL-35, which individually are required for maximal Treg activity. Surprisingly, IL-10/IL-35-double deficient Tregswere fully functionalin vitro and in vivo. Loss of IL-10 and IL-35 was compensated for by a concurrent increase in cathepsin E (CTSE) expression, enhanced TRAIL (Tnfsf10)expression and soluble TRAIL release, rendering IL-10/IL-35-double deficient Tregsfunctionally dependent on TRAIL in vitro and in vivo. Lastly, while C57BL/6 Tregs are IL-10/IL-35-dependent, Balb/c Tregs, which express high levels of CTSE and enhanced TRAIL expression, are TRAIL-dependent.These data reveal that cross-regulatory pathways exist, which control the utilization of suppressive mechanisms,thereby providing Tregfunctional plasticity. Isolate natural Tregs from the different knockout mouse
- Species:
- mouse
- Samples:
- 12
- Source:
- E-GEOD-29262
- PubMed:
- 22013112
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | GENOTYPE | BATCH |
---|---|---|
GSM723290 | Wild type Treg | 1 |
GSM72329 | Ebi3 –/– Treg | 1 |
GSM723292 | Ebi3 –/– Il10 –/– Treg | 1 |
GSM723293 | Il10 –/– Treg | 1 |
GSM723294 | Wild type Treg | 2 |
GSM723295 | Ebi3 –/– Treg | 2 |
GSM723296 | Il10 –/– Treg | 2 |
GSM723297 | Ebi3 –/– Il10 –/– Treg | 2 |
GSM723298 | Wild type Treg | 3 |
GSM723299 | Il10 –/– Treg | 3 |
GSM723300 | Ebi3 –/– Treg | 3 |
GSM72330 | Ebi3 –/– Il10 –/– Treg | 3 |