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Home › Dataset Library › Ath29 congenic mice - gene expression in aorta

Dataset: Ath29 congenic mice - gene expression in aorta

A congenic mouse line was constructed by introgressing a C3H chromosome 9 region harboring Ath29 into the C57BL/6 apoE-deficient...

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A congenic mouse line was constructed by introgressing a C3H chromosome 9 region harboring Ath29 into the C57BL/6 apoE-deficient background. RNA was extracted from aorta using a QIAGEN kit . Total RNA was pooled in an equal amount from 3 mice for each group. Standard Affymetrix procedures were performed using 8ug of total RNA. Microarrays were used to detect gene expression in the aorta of Ath29 congenic mice and C57BL/6 apoE-deficient mouse strains fed a chow or western diet. 4 groups of mice were studied: C57BL/6 apoE-/- control mice and Ath29 congenic mice fed a chow or Western diet. After fed a Western diet for 2 weeks, aorta was harvested and total RNA prepared.

Species:
mouse

Samples:
4

Source:
E-GEOD-29149

Updated:
Dec.12, 2014

Registered:
Nov.11, 2014


Factors: (via ArrayExpress)
Sample DISEASE STATE GENOTYPE DIET
GSM721548 Resistance to atherosclerosis Ath29 congenic mice chow
GSM721549 Atherosclerosis C57BL/6 apoE-/- mice chow
GSM721550 Resistance to atherosclerosis Ath29 congenic mice western
GSM72155 Atherosclerosis C57BL/6 apoE-/- mice western

Tags

  • aorta
  • chromosome
  • line

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