Dataset: Transcription profiling of microdissected and hand sorted mouse forebrain neurons to understand the extend and nature of differential expression in various cell types.

The mammalian forebrain is a tissue of stunning complexity comprised of numerous regions each containing many distinct cell types that differ in their intrinsic and synaptic physiology, morphology and connectivity. These differences are likely conferred by differential gene expression, but the extent and nature of cell type specific gene expression is largely unknown. Here, we carried out microarray analysis of twelve major classes of fluorescently labelled neurons within the forebrain and provide the first comprehensive view of gene expression differences. The results demonstrate a profound molecular heterogeneity among neuronal subtypes, represented disproportionately by gene paralogs, and begin to reveal the genetic programs underlying the fundamental divisions between neuronal classes including that between glutamatergic and GABAergic neurons. Experiment Overall Design: Total of 36 samples from 12 different cell types were anlyzed. Three biological replicates (each sample from different animal(s)) were analyzed for each cell type.

Species:

mouse

Samples:

42

Source:

E-GEOD-2882

PubMed:

16369481

Updated:

Dec.12, 2014

Registered:

Nov.24, 2014