Dataset: PPARg and IL-4-induced gene expression data from wild-type and STAT6 knockout mouse bone marrow-derived macrophages
C57Bl/6 wild-type and STAT6 KO mice were used to study PPARg and IL-4 signaling. Bone marrow of 3 mice per group was isolated and...
C57Bl/6 wild-type and STAT6 KO mice were used to study PPARg and IL-4 signaling. Bone marrow of 3 mice per group was isolated and differentiated to macrophages with M-CSF (20 ng/ml). 20 ng/ml IL-4 was used to induce alternative macrophage activation and 1 uM Rosiglitazone (RSG) was used to activate PPARg. From each mouse 4 samples were generated: 1. M-CSF, 2. M-CSF+RSG, 3. IL-4 and 4. IL-4+RSG. All compounds were added throughout the whole differentiation process, and frech media was added every other day. Control cells were treated with vehicle (DMSO:ethanol). After 10 days, RNA was isolated and gene expression profiles were analyzed using Mouse Genome 430 2.0 microarrays from Affymetrix. 3 C57Bl/6 wild-type and 3 STAT6 KO mice were used to isolate bone marrow and from each macrophages were differentiated with or without IL-4 and simultaneously treated with vehicle or RSG. Altogether we analyzed 24 samples with 3 biological replicates as below.
- Species:
- mouse
- Samples:
- 24
- Source:
- E-GEOD-25088
- PubMed:
- 21093321
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | ACTIVATION | MOUSE | TREATMENT | VARIATION |
---|---|---|---|---|
GSM616130 | control | 1 | control | Wild-type |
GSM61613 | control | 1 | rosiglitazone | Wild-type |
GSM616132 | IL-4 | 1 | control | Wild-type |
GSM616133 | IL-4 | 1 | rosiglitazone | Wild-type |
GSM616134 | control | 2 | control | Wild-type |
GSM616135 | control | 2 | rosiglitazone | Wild-type |
GSM616136 | IL-4 | 2 | control | Wild-type |
GSM616137 | IL-4 | 2 | rosiglitazone | Wild-type |
GSM616138 | control | 3 | control | Wild-type |
GSM616139 | control | 3 | rosiglitazone | Wild-type |
GSM616140 | IL-4 | 3 | control | Wild-type |
GSM61614 | IL-4 | 3 | rosiglitazone | Wild-type |
GSM616142 | control | 4 | control | STAT6 KO |
GSM616143 | control | 4 | rosiglitazone | STAT6 KO |
GSM616144 | IL-4 | 4 | control | STAT6 KO |
GSM616145 | IL-4 | 4 | rosiglitazone | STAT6 KO |
GSM616146 | control | 5 | control | STAT6 KO |
GSM616147 | control | 5 | rosiglitazone | STAT6 KO |
GSM616148 | IL-4 | 5 | control | STAT6 KO |
GSM616149 | IL-4 | 5 | rosiglitazone | STAT6 KO |
GSM616150 | control | 6 | control | STAT6 KO |
GSM61615 | control | 6 | rosiglitazone | STAT6 KO |
GSM616152 | IL-4 | 6 | control | STAT6 KO |
GSM616153 | IL-4 | 6 | rosiglitazone | STAT6 KO |