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Home › Dataset Library › Expression data from differentiating ES cells expressing miR-200c and miR-141

Dataset: Expression data from differentiating ES cells expressing miR-200c and miR-141

ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell...

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ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline. A2.miR200c ES cells, which express miR-200c/miR-141 upon addition of doxycycline, were differentiated as embryoid bodies in six petri dishes. Half of the samples were treated with doxycycline on days 2 and 4 of differentiation, and RNA was collected from all samples on days 3, 4, and 5.

Species:
mouse

Samples:
6

Source:
E-GEOD-24289

Updated:
Dec.12, 2014

Registered:
Nov.11, 2014


Factors: (via ArrayExpress)
Sample PROTOCOL AGENT TIME
GSM597445 control untreated day 3 of differentiation
GSM597446 inducible expression of miR-200c/miR-141 doxycycline day 3 of differentiation
GSM597447 control untreated day 4 of differentiation
GSM597448 inducible expression of miR-200c/miR-141 doxycycline day 4 of differentiation
GSM597449 control untreated day 5 of differentiation
GSM597450 inducible expression of miR-200c/miR-141 doxycycline day 5 of differentiation

Tags

  • cell
  • line
  • serum

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