Dataset: Expression data from differentiating ES cells expressing miR-200c and miR-141
ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell...
ES cells express the miR-200 family which becomes down-regulated during the course of differentiation in serum. We generated an ES cell line which expresses miR-200c and miR-141 upon addition of doxycycline. Microarrays were used to gain a global picture of differentiation when miR-200c and miR-141 expression were maintained throughout differentiation through the addition of doxycycline. A2.miR200c ES cells, which express miR-200c/miR-141 upon addition of doxycycline, were differentiated as embryoid bodies in six petri dishes. Half of the samples were treated with doxycycline on days 2 and 4 of differentiation, and RNA was collected from all samples on days 3, 4, and 5.
- Species:
- mouse
- Samples:
- 6
- Source:
- E-GEOD-24289
- Updated:
- Dec.12, 2014
- Registered:
- Nov.11, 2014
Sample | PROTOCOL | AGENT | TIME |
---|---|---|---|
GSM597445 | control | untreated | day 3 of differentiation |
GSM597446 | inducible expression of miR-200c/miR-141 | doxycycline | day 3 of differentiation |
GSM597447 | control | untreated | day 4 of differentiation |
GSM597448 | inducible expression of miR-200c/miR-141 | doxycycline | day 4 of differentiation |
GSM597449 | control | untreated | day 5 of differentiation |
GSM597450 | inducible expression of miR-200c/miR-141 | doxycycline | day 5 of differentiation |