Dataset: Transcriptome profiling of genes regulated by RXR and its partners in monocyte-derived dendritic cells
CD14+ human monocytes differentiating into DCs in the presence of IL4 and GM-CSF were treated with agonists for RXR and its partners or...
CD14+ human monocytes differentiating into DCs in the presence of IL4 and GM-CSF were treated with agonists for RXR and its partners or vehicle 18 hours after plating (experiment with RXR and permissive partners, donor 1-3) or 14 hours after plating (experiment with nonpermissive partners, donor 4-6). Cells were harvested 12 hours thereafter. Experiments were performed in biological triplicates representing samples from three different donors. In this study all probable RXR-signaling pathways induced by agonists for RXR, LXRs, PPARs, RAR and VDR were identified in differentiating human monocyte-derived dendritic cells. In the experiments, differentiating dendritic cells were treated for 12 hours with one of the following compounds (ligands): vehicle (DMS:EtOH 1:1) LG268 (RXR agonist) 9-cis retinoic acid (9cisRA, agonist of RAR and RXR) GW3965 (LXRalpha/beta panagonist) rosiglitazone (RSG, PPARgamma agonist) GW1516 (PPARdelta agonist) AM580 (RARalpha agonist) 1,25-dihydroxyvitamin D3 (VDR agonist)
- Species:
- human
- Samples:
- 27
- Source:
- E-GEOD-23073
- PubMed:
- 20861222
- Updated:
- Dec.12, 2014
- Registered:
- Sep.15, 2014
| Sample | NUCLEAR RECEPTOR AGONIST TYPE | DONOR | AGENT |
|---|---|---|---|
| GSM568964 | control | 1 | vehicle |
| GSM568965 | RAR and RXR agonist | 1 | 9-cis retinoic acid |
| GSM568966 | RXR agonist | 1 | LG268 |
| GSM568967 | PPARg agonist | 1 | rosiglitazone |
| GSM568968 | PPARd agonist | 1 | GW1516 |
| GSM568969 | LXR agonist | 1 | GW3965 |
| GSM568970 | control | 2 | vehicle |
| GSM56897 | RAR and RXR agonist | 2 | 9-cis retinoic acid |
| GSM568972 | RXR agonist | 2 | LG268 |
| GSM568973 | PPARg agonist | 2 | rosiglitazone |
| GSM568974 | PPARd agonist | 2 | GW1516 |
| GSM568975 | LXR agonist | 2 | GW3965 |
| GSM568976 | control | 3 | vehicle |
| GSM568977 | RAR and RXR agonist | 3 | 9-cis retinoic acid |
| GSM568978 | RXR agonist | 3 | LG268 |
| GSM568979 | PPARg agonist | 3 | rosiglitazone |
| GSM568980 | PPARd agonist | 3 | GW1516 |
| GSM56898 | LXR agonist | 3 | GW3965 |
| GSM568982 | control | 4 | vehicle |
| GSM568983 | RAR agonist | 4 | AM580 |
| GSM568984 | VDR agonist | 4 | 1,25-dihydroxyvitamin D3 |
| GSM568985 | control | 5 | vehicle |
| GSM568986 | RAR agonist | 5 | AM580 |
| GSM568987 | VDR agonist | 5 | 1,25-dihydroxyvitamin D3 |
| GSM568988 | control | 6 | vehicle |
| GSM568989 | RAR agonist | 6 | AM580 |
| GSM568990 | VDR agonist | 6 | 1,25-dihydroxyvitamin D3 |