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Home › Dataset Library › Gene expression data from somites of E9.5 mouse embryos

Dataset: Gene expression data from somites of E9.5 mouse embryos

We used microarrays to identify Pax3 targets during myogenesis in the mouse embryo Mouse embryos were genotyped Pax3GFP/+ or...

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We used microarrays to identify Pax3 targets during myogenesis in the mouse embryo Mouse embryos were genotyped Pax3GFP/+ or Pax3PAX3-FKHR/GFP and dissected at E9.5 under a fluorescent binocular. Somites were dissected from the interlimb region and the more hypaxial domain separated from the neural tube and the epaxial extremity of the somites. GFP positive cells were then sorted by flow cytometry before RNA extraction and hybridization on Affymetrix microarrays. We also sorted GFP negative cells.

Species:
mouse

Samples:
9

Source:
E-GEOD-22040

Updated:
Dec.12, 2014

Registered:
Nov.11, 2014


Factors: (via ArrayExpress)
Sample GENOTYPE/VARIATION
GSM547860 Pax3PAX3-FKHR/GFP
GSM547860 Pax3PAX3-FKHR/GFP
GSM547860 Pax3PAX3-FKHR/GFP
GSM547863 Pax3GFP/+
GSM547863 Pax3GFP/+
GSM547863 Pax3GFP/+
GSM547866 GFP-
GSM547866 GFP-
GSM547866 GFP-

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  • embryo

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