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Home › Dataset Library › Effects of gene scilencing of USP2 on expression profiles of HL60 cells

Dataset: Effects of gene scilencing of USP2 on expression profiles of HL60 cells

So far, we have found PMA induced USP2b isoform in myeloid leukemia cell lines such as HL60, THP-1, and U937. To explore molecular...

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So far, we have found PMA induced USP2b isoform in myeloid leukemia cell lines such as HL60, THP-1, and U937. To explore molecular function of USP2 in the cells, we assess expression profiles of HL60-derivatives continuously expressing shRNA for USP2 and control shRNA. HL60 derivatives continuously expressing shRNA for USP2 and control shRNA were treated with PMA (30nM) for 0, 1, 2, and 3 days. Total RNA was extracted from the cells and subjected to microarray experiments using Human Genome U133 GeneChips.

Species:
human

Samples:
24

Source:
E-GEOD-20476

Updated:
Dec.12, 2014

Registered:
Jul.12, 2014


Factors: (via ArrayExpress)
Sample DURATION TRANSDUCTION PRETREATMENTDOSE
GSM513728 3 days negative control shRNA 32nM
GSM513728 3 days negative control shRNA 32nM
GSM513728 3 days negative control shRNA 32nM
GSM513725 2 days negative control shRNA 32nM
GSM513725 2 days negative control shRNA 32nM
GSM513725 2 days negative control shRNA 32nM
GSM513722 1 day negative control shRNA 32nM
GSM513722 1 day negative control shRNA 32nM
GSM513722 1 day negative control shRNA 32nM
GSM513719 0 day negative control shRNA 0nM
GSM513719 0 day negative control shRNA 0nM
GSM513719 0 day negative control shRNA 0nM
GSM513716 3 days USP2 shRNA 32nM
GSM513716 3 days USP2 shRNA 32nM
GSM513716 3 days USP2 shRNA 32nM
GSM513713 2 days USP2 shRNA 32nM
GSM513713 2 days USP2 shRNA 32nM
GSM513713 2 days USP2 shRNA 32nM
GSM513710 1 day USP2 shRNA 32nM
GSM513710 1 day USP2 shRNA 32nM
GSM513710 1 day USP2 shRNA 32nM
GSM513707 0 day USP2 shRNA 0nM
GSM513707 0 day USP2 shRNA 0nM
GSM513707 0 day USP2 shRNA 0nM

Tags

  • cell
  • genome
  • leukemia
  • myeloid leukemia

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