Dataset: Transcriptome Analysis and Molecular Signature of Human Retinal Pigment Epithelium

[u'The retinal pigment epithelium (RPE) is a polarized cell layer that is critical for photoreceptor function and survival. It\u2019s unique relationship to the photoreceptors and its specific physiology makes the RPE a critical determinant of human vision. Therefore we performed global expression profiling of native and cultured human fetal and adult RPE and determined a unique set of highly-expressed genes (called the \u201csignature\u201d set) by comparing the observed RPE gene profiles to the Novartis expression database (SymAtlas: ', {u'a': {u'href': u'http://wombat.gnf.org/index.html', u'target': u'_blank', u'$': u'http://wombat.gnf.org/index.html'}}, u') of 78 tissues. A comparative analysis of transcriptomes from human fetal and adult RPE, primary cultures and commonly-used cell lines was performed. Using selection criteria of at least 10-fold higher expression in each of three RPE preparations, we identified 154 RPE signature genes, which were validated by qRT-PCR analysis in RPE and in an independent set of 11 tissues.']

Species:

human

Samples:

31

Source:

E-GEOD-18811

PubMed:

20360305

Updated:

Dec.12, 2014

Registered:

Sep.15, 2014