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Home › Dataset Library › TGF or TNF Time series in ARPE19

Dataset: TGF or TNF Time series in ARPE19

Aberrant epithelial-mesenchymal transition (EMT) is involved in pathological processes including fibrotic disorders and cancer invasion...

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Aberrant epithelial-mesenchymal transition (EMT) is involved in pathological processes including fibrotic disorders and cancer invasion and metastasis. Alterations of the cell-extracellular matrix (ECM) interaction also contribute to those pathological settings. However, the functional interplay between EMT and cell-ECM interaction is poorly understood. Here, we show that tumor necrosis factor (TNF)-α, a potent mediator of inflammation, induces EMT-associated fibrosis in retinal pigment epithelial cells, and that this is regulated by hyaluronan (HA)-CD44-Moesin interaction. TNF-α elicits both HA synthesis and Moesin phosphorylation through protein kinase C activation, promoting binding of CD44 to the newly synthesized HA. The HA-CD44-Moesin interaction leads to cell-cell dissociation through actin remodeling and increased cellular motility associated with mesenchymal phenotype. Furthermore, we established an in vivo model of TNF-α-induced fibrosis in the mouse eye, and the ocular fibrosis was completely suppressed in CD44-null mice. Therefore, HA production and its interaction with CD44 plays essential role in TNF-α-induced-EMT, and the interference of the complex formation can be a new strategy for the fibrotic disorders. ARPE19 cell lines were treated with TGF and TNF for 6 and 42 hour. Each experiment were repeated three times. But 1hour experiment was repeated two times. For this submission, total RNA was extracted from TGF- or TNF-treated ARPE-19 cells and differential gene expression between each time point (6 and 42 hours) was determined using genechip arrays (Affymetrix, Human Genome U133).

Species:
human

Samples:
12

Source:
E-GEOD-15205

Updated:
Dec.12, 2014

Registered:
Sep.12, 2014


Factors: (via ArrayExpress)
Sample AGENT TIME
GSM379690 TGF 6 hours
GSM379690 TGF 6 hours
GSM379690 TGF 6 hours
GSM379693 TNF 6 hours
GSM379693 TNF 6 hours
GSM379693 TNF 6 hours
GSM379696 TGF 42 hours
GSM379696 TGF 42 hours
GSM379696 TGF 42 hours
GSM379699 TNF 42 hours
GSM379699 TNF 42 hours
GSM379699 TNF 42 hours

Tags

  • actin
  • cancer
  • cell
  • eye
  • genome
  • hyaluronan
  • point
  • protein

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