{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM379690": {"AGENT": "TGF", "TIME": "6 hours"}}, {"GSM379690": {"AGENT": "TGF", "TIME": "6 hours"}}, {"GSM379690": {"AGENT": "TGF", "TIME": "6 hours"}}, {"GSM379693": {"AGENT": "TNF", "TIME": "6 hours"}}, {"GSM379693": {"AGENT": "TNF", "TIME": "6 hours"}}, {"GSM379693": {"AGENT": "TNF", "TIME": "6 hours"}}, {"GSM379696": {"AGENT": "TGF", "TIME": "42 hours"}}, {"GSM379696": {"AGENT": "TGF", "TIME": "42 hours"}}, {"GSM379696": {"AGENT": "TGF", "TIME": "42 hours"}}, {"GSM379699": {"AGENT": "TNF", "TIME": "42 hours"}}, {"GSM379699": {"AGENT": "TNF", "TIME": "42 hours"}}, {"GSM379699": {"AGENT": "TNF", "TIME": "42 hours"}}], "id": 3209, "pop_total": 0, "platform": 4, "summary_wrapped": "Aberrant epithelial-mesenchymal transition (EMT) is involved in\u3000pathological processes including fibrotic disorders and cancer invasion...", "geo_gse_id": "E-GEOD-15205", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["actin", "cancer", "cell", "eye", "genome", "hyaluronan", "point", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-15205_A-AFFY-44", "slug": "tgf-or-tnf-time-series-in-arpe19", "geo_gds_id": "", "name": "TGF or TNF Time series in ARPE19", "created": "Sep.12, 2014", "summary": "Aberrant epithelial-mesenchymal transition (EMT) is involved in\u3000pathological processes including fibrotic disorders and cancer invasion and metastasis. Alterations of the cell-extracellular matrix\u3000(ECM) interaction also contribute to those pathological settings.\u3000However, the functional interplay between EMT and cell-ECM\u3000interaction is poorly understood. Here, we show that tumor necrosis factor (TNF)-\u03b1, a potent mediator of inflammation, induces\u3000EMT-associated fibrosis in retinal pigment epithelial cells, and that this is regulated by hyaluronan (HA)-CD44-Moesin interaction. TNF-\u03b1 elicits both HA synthesis and Moesin phosphorylation through protein kinase C activation, promoting binding of CD44 to the newly synthesized HA. The HA-CD44-Moesin interaction leads to cell-cell dissociation through actin remodeling and increased cellular motility associated with mesenchymal phenotype. Furthermore, we\u3000established an in vivo model of TNF-\u03b1-induced fibrosis in the mouse eye, and the ocular fibrosis was completely suppressed in CD44-null mice. Therefore, HA production and its interaction with CD44 plays essential role in TNF-\u03b1-induced-EMT, and the interference of the complex formation can be a new strategy for the fibrotic disorders. ARPE19 cell lines were treated with TGF and TNF for 6 and 42 hour. Each experiment were repeated three times. But 1hour experiment was repeated two times. For this submission, total RNA was extracted from TGF- or TNF-treated ARPE-19 cells and differential gene expression between each time point (6 and 42 hours) was determined using genechip arrays (Affymetrix, Human Genome U133).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15205", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-15205/samples/"}