Dataset: Laser capture microdissection of endothelial and neuronal cells from human dorsolateral prefrontal cortex

We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from...

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We used laser capture microdissection to isolate both microvascular endothelial cells and neurons from post mortem brain tissue from patients with schizophrenia and bipolar disorder and healthy controls. RNA was isolated from these cell populations, amplified, and analysed using Affymetrix HG133plus2.0 GeneChips. In the first instance, we used the dataset to compare the neuronal and endothelial data, in order to demonstrate that the predicted differences between cell types could be detected using this methodology. Keywords: cell type comparison, laser capture microdissection The dataset consists of endothelial cell samples from 18 individuals and neuronal samples from an overlapping population of 18 individuals.

Species:: human
Samples:: 32
Source:: E-GEOD-12679
PubMed:: 19088852
Updated:: Dec.12, 2014
Registered:: Sep.10, 2014

Factors: (via ArrayExpress)

Sample	PMI	AGE	DIAGNOSTIC GROUP	CELL TYPE	SEX
GSM318410	35h	45	schizophrenia	endothelial	male
GSM3184	30h	37	schizophrenia	endothelial	male
GSM318412	35h	45	schizophrenia	neuronal	male
GSM318413	30h	37	schizophrenia	neuronal	male
GSM318414	37h	42	schizophrenia	endothelial	male
GSM318415	13h	32	control	endothelial	male
GSM318416	9h	44	schizophrenia	endothelial	male
GSM318417	38h	54	schizophrenia	endothelial	male
GSM318418	18h	45	schizophrenia	endothelial	male
GSM318419	10h	44	control	endothelial	female
GSM318420	28h	19	schizophrenia	endothelial	male
GSM31842	35h	47	schizophrenia	endothelial	female
GSM318422	42h	54	schizophrenia	endothelial	female
GSM318423	30h	47	schizophrenia	endothelial	female
GSM318424	19h	42	schizophrenia	endothelial	male
GSM318425	11h	47	control	endothelial	male
GSM318426	31h	55	control	endothelial	male
GSM318427	22h	34	control	endothelial	male
GSM318428	11h	47	control	neuronal	male
GSM318429	58h	39	control	neuronal	female
GSM318430	26h	44	schizophrenia	neuronal	female
GSM31843	28h	41	bipolar disorder	neuronal	female
GSM318432	23h	48	bipolar disorder	neuronal	male
GSM318433	31h	46	control	neuronal	male
GSM318434	35h	35	bipolar disorder	neuronal	male
GSM318435	23h	51	bipolar disorder	neuronal	male
GSM318436	13h	32	control	neuronal	male
GSM318437	32h	42	bipolar disorder	neuronal	male
GSM318438	16h	52	schizophrenia	neuronal	male
GSM318439	35h	47	schizophrenia	neuronal	female
GSM318440	22h	34	control	neuronal	male
GSM31844	45h	49	control	neuronal	female