Dataset: Diverse human extracellular RNAs are widely detected in human plasma.

Extracellular RNA from the plasma of 40 individuals from the Framingham Heart Study were isolated using miRCURY RNA isolation kit...

Registered by ERCC

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Extracellular RNA from the plasma of 40 individuals from the Framingham Heart Study were isolated using miRCURY RNA isolation kit Biofluids (Exiqon). Small RNA Library preparation was done with Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and the samples were sequenced with the Ion Proton. Next, using a targeted quantitative PCR with reverse transcription approach in 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.

Species:: human
Samples:: 39
PubMed:: 27112789
Updated:: Jul.30, 2018
Registered:: Jul.30, 2018

Factors:

Sample	trancriptome_genome-ratio	cell_culture_source	related_dataset_id	fastq_identifier	biosample_metadata_id	exRNA_source	transcriptome_reads	species	condition	biofluid_name	RNA_isolation_kit	profiling_assay	meets_ERCC_QC	reference_genome_reads	anatomical_location
11020	0.896134	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1020_CVD_BC_12_fastq	EXR-JFREE11020-BS	total cell-free biofluid RNA	542,550	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	605,434	Entire cardiovascular system
11066	0.866619	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1066_No_CVD_BC_11_fastq	EXR-JFREE11066-BS	total cell-free biofluid RNA	269,652	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	311,154	Entire cardiovascular system
11095	0.86556	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1095_No_CVD_BC_13_fastq	EXR-JFREE11095-BS	total cell-free biofluid RNA	333,771	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	385,613	Entire cardiovascular system
11120	0.906389	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1120_No_CVD_BC_01_fastq	EXR-JFREE11120-BS	total cell-free biofluid RNA	166,607	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	183,814	Entire cardiovascular system
11430	0.8688	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1430_CVD_BC_10_fastq	EXR-JFREE11430-BS	total cell-free biofluid RNA	731,229	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	841,645	Entire cardiovascular system
11579	0.746617	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1579_CVD_BC_10_fastq	EXR-JFREE11579-BS	total cell-free biofluid RNA	183,753	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	246,114	Entire cardiovascular system
11610	0.93647	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_1610_CVD_BC_04_fastq	EXR-JFREE11610-BS	total cell-free biofluid RNA	1,007,685	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,076,046	Entire cardiovascular system
10172	0.768581	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_172_CVD_BC_14_fastq	EXR-JFREE10172-BS	total cell-free biofluid RNA	276,109	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	359,245	Entire cardiovascular system
10216	0.945584	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_216_N0_CVD_BC_03_fastq	EXR-JFREE10216-BS	total cell-free biofluid RNA	1,500,424	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,586,770	Entire cardiovascular system
10021	0.83462	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_21_No_CVD_BC_01_fastq	EXR-JFREE10021-BS	total cell-free biofluid RNA	210,235	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	251,893	Entire cardiovascular system
12303	0.788773	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_2303_No_CVD_BC_07_fastq	EXR-JFREE12303-BS	total cell-free biofluid RNA	211,627	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	268,299	Entire cardiovascular system
12616	0.790398	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_2616_No_CVD_BC_05_fastq	EXR-JFREE12616-BS	total cell-free biofluid RNA	130,701	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	165,361	Entire cardiovascular system
12768	0.953922	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_2768_CVD_BC_02_fastq	EXR-JFREE12768-BS	total cell-free biofluid RNA	1,249,385	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,309,735	Entire cardiovascular system
12918	0.938385	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_2918_No_CVD_BC01_fastq	EXR-JFREE12918-BS	total cell-free biofluid RNA	1,095,028	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,166,928	Entire cardiovascular system
12938	0.716815	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_2938_CVD_BC_04_fastq	EXR-JFREE12938-BS	total cell-free biofluid RNA	88,318	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	123,209	Entire cardiovascular system
13132	0.826711	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_3132_N0_CVD_BC_05_fastq	EXR-JFREE13132-BS	total cell-free biofluid RNA	177,265	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	214,422	Entire cardiovascular system
13170	0.751055	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_3170_No_CVD_BC_07_fastq	EXR-JFREE13170-BS	total cell-free biofluid RNA	76,519	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	101,882	Entire cardiovascular system
10320	0.946026	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_320_No_CVD_BC_07_fastq	EXR-JFREE10320-BS	total cell-free biofluid RNA	812,926	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	859,306	Entire cardiovascular system
13392	0.838254	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_3392_CVD_BC_02_fastq	EXR-JFREE13392-BS	total cell-free biofluid RNA	243,884	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	290,943	Entire cardiovascular system
13464	0.713995	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_3464_No_CVD_BC_03_fastq	EXR-JFREE13464-BS	total cell-free biofluid RNA	59,670	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	83,572	Entire cardiovascular system
13713	0.822151	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_3713_CVD_BC_06_fastq	EXR-JFREE13713-BS	total cell-free biofluid RNA	148,945	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	181,165	Entire cardiovascular system
14043	0.834504	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_4043_CVD_BC_04_fastq	EXR-JFREE14043-BS	total cell-free biofluid RNA	177,837	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	213,105	Entire cardiovascular system
14820	0.80196	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_4820_No_CVD_BC_13_fastq	EXR-JFREE14820-BS	total cell-free biofluid RNA	175,885	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	219,319	Entire cardiovascular system
14912	0.701753	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_4912_CVD_BC_02_fastq	EXR-JFREE14912-BS	total cell-free biofluid RNA	46,449	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	66,190	Entire cardiovascular system
10500	0.689864	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_500_No_CVD_BC_09_fastq	EXR-JFREE10500-BS	total cell-free biofluid RNA	90,586	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	131,310	Entire cardiovascular system
15237	0.822248	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_5237_CVD_BC_06_fastq	EXR-JFREE15237-BS	total cell-free biofluid RNA	151,001	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	183,644	Entire cardiovascular system
10543	0.757923	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_543_CVD_BC_16_fastq	EXR-JFREE10543-BS	total cell-free biofluid RNA	91,811	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	NO	121,135	Entire cardiovascular system
10590	0.842146	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_590_N0_CVD_BC_15_fastq	EXR-JFREE10590-BS	total cell-free biofluid RNA	150,126	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	178,266	Entire cardiovascular system
16077	0.953763	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6077_No_CVD_BC_09_fastq	EXR-JFREE16077-BS	total cell-free biofluid RNA	1,378,861	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,445,706	Entire cardiovascular system
16136	0.825893	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6136_CVD_BC_08_fastq	EXR-JFREE16136-BS	total cell-free biofluid RNA	156,354	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	189,315	Entire cardiovascular system
16355	0.908901	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6355_CVD_BC_08_fastq	EXR-JFREE16355-BS	total cell-free biofluid RNA	727,498	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	800,415	Entire cardiovascular system
16684	0.825828	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6684_CVD_BC_16_fastq	EXR-JFREE16684-BS	total cell-free biofluid RNA	294,681	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	356,831	Entire cardiovascular system
16758	0.766947	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6758_CVD_BC_14_fastq	EXR-JFREE16758-BS	total cell-free biofluid RNA	185,579	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	241,971	Entire cardiovascular system
10676	0.943756	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_676_CVD_BC_06_fastq	EXR-JFREE10676-BS	total cell-free biofluid RNA	1,031,504	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	1,092,977	Entire cardiovascular system
16926	0.868186	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_6926_No_CVD_BC_03_fastq	EXR-JFREE16926-BS	total cell-free biofluid RNA	178,532	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	205,638	Entire cardiovascular system
17138	0.804494	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_7138_CVD_BC_12_fastq	EXR-JFREE17138-BS	total cell-free biofluid RNA	295,317	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	367,084	Entire cardiovascular system
17226	0.765965	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_7226_No_CVD_BC_15_fastq	EXR-JFREE17226-BS	total cell-free biofluid RNA	466,364	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	608,858	Entire cardiovascular system
10728	0.770573	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_728_CVD_BC_08_fastq	EXR-JFREE10728-BS	total cell-free biofluid RNA	128,725	Human	Cardiovascular Disorder	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	167,051	Entire cardiovascular system
17326	0.750356	Not Applicable	EXR-JFREE1eZDUKB-AN	sample_FHS_7326_No_CVD_BC_11_fastq	EXR-JFREE17326-BS	total cell-free biofluid RNA	139,705	Human	Healthy Control	Plasma	miRcury biofluids (Exiqon)	small RNA-Seq	YES	186,185	Entire cardiovascular system