Dataset: Diverse human extracellular RNAs are widely detected in human plasma.

Extracellular RNA from the plasma of 40 individuals from the Framingham Heart Study were isolated using miRCURY RNA isolation kit...

Registered by ERCC

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Extracellular RNA from the plasma of 40 individuals from the Framingham Heart Study were isolated using miRCURY RNA isolation kit Biofluids (Exiqon). Small RNA Library preparation was done with Ion Total RNA-Seq Kit v2 (Thermo Fisher Scientific) and the samples were sequenced with the Ion Proton. Next, using a targeted quantitative PCR with reverse transcription approach in 2,763 individuals, we characterized almost 500 of the most abundant extracellular transcripts including microRNAs, piRNAs and small nucleolar RNAs. The presence in plasma of many non-microRNA small-RNAs was confirmed in an independent cohort. We present comprehensive data to demonstrate the broad and consistent detection of diverse classes of circulating non-cellular small-RNAs from a large population.

Species:: human
Samples:: 39
PubMed:: 27112789
Updated:: Jul.30, 2018
Registered:: Jul.30, 2018

Factors:

Sample	trancriptome_genome-ratio	transcriptome_reads	related_dataset_id	fastq_identifier	biosample_metadata_id	exRNA_source	cell_culture_source	reference_genome_reads	condition	RNA_isolation_kit	profiling_assay	meets_ERCC_QC	species	anatomical_location	biofluid_name
11020	0.896134	542,550	EXR-JFREE1eZDUKB-AN	sample_FHS_1020_CVD_BC_12_fastq	EXR-JFREE11020-BS	total cell-free biofluid RNA	Not Applicable	605,434	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11066	0.866619	269,652	EXR-JFREE1eZDUKB-AN	sample_FHS_1066_No_CVD_BC_11_fastq	EXR-JFREE11066-BS	total cell-free biofluid RNA	Not Applicable	311,154	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11095	0.86556	333,771	EXR-JFREE1eZDUKB-AN	sample_FHS_1095_No_CVD_BC_13_fastq	EXR-JFREE11095-BS	total cell-free biofluid RNA	Not Applicable	385,613	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11120	0.906389	166,607	EXR-JFREE1eZDUKB-AN	sample_FHS_1120_No_CVD_BC_01_fastq	EXR-JFREE11120-BS	total cell-free biofluid RNA	Not Applicable	183,814	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11430	0.8688	731,229	EXR-JFREE1eZDUKB-AN	sample_FHS_1430_CVD_BC_10_fastq	EXR-JFREE11430-BS	total cell-free biofluid RNA	Not Applicable	841,645	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11579	0.746617	183,753	EXR-JFREE1eZDUKB-AN	sample_FHS_1579_CVD_BC_10_fastq	EXR-JFREE11579-BS	total cell-free biofluid RNA	Not Applicable	246,114	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
11610	0.93647	1,007,685	EXR-JFREE1eZDUKB-AN	sample_FHS_1610_CVD_BC_04_fastq	EXR-JFREE11610-BS	total cell-free biofluid RNA	Not Applicable	1,076,046	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10172	0.768581	276,109	EXR-JFREE1eZDUKB-AN	sample_FHS_172_CVD_BC_14_fastq	EXR-JFREE10172-BS	total cell-free biofluid RNA	Not Applicable	359,245	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10216	0.945584	1,500,424	EXR-JFREE1eZDUKB-AN	sample_FHS_216_N0_CVD_BC_03_fastq	EXR-JFREE10216-BS	total cell-free biofluid RNA	Not Applicable	1,586,770	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10021	0.83462	210,235	EXR-JFREE1eZDUKB-AN	sample_FHS_21_No_CVD_BC_01_fastq	EXR-JFREE10021-BS	total cell-free biofluid RNA	Not Applicable	251,893	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
12303	0.788773	211,627	EXR-JFREE1eZDUKB-AN	sample_FHS_2303_No_CVD_BC_07_fastq	EXR-JFREE12303-BS	total cell-free biofluid RNA	Not Applicable	268,299	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
12616	0.790398	130,701	EXR-JFREE1eZDUKB-AN	sample_FHS_2616_No_CVD_BC_05_fastq	EXR-JFREE12616-BS	total cell-free biofluid RNA	Not Applicable	165,361	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
12768	0.953922	1,249,385	EXR-JFREE1eZDUKB-AN	sample_FHS_2768_CVD_BC_02_fastq	EXR-JFREE12768-BS	total cell-free biofluid RNA	Not Applicable	1,309,735	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
12918	0.938385	1,095,028	EXR-JFREE1eZDUKB-AN	sample_FHS_2918_No_CVD_BC01_fastq	EXR-JFREE12918-BS	total cell-free biofluid RNA	Not Applicable	1,166,928	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
12938	0.716815	88,318	EXR-JFREE1eZDUKB-AN	sample_FHS_2938_CVD_BC_04_fastq	EXR-JFREE12938-BS	total cell-free biofluid RNA	Not Applicable	123,209	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
13132	0.826711	177,265	EXR-JFREE1eZDUKB-AN	sample_FHS_3132_N0_CVD_BC_05_fastq	EXR-JFREE13132-BS	total cell-free biofluid RNA	Not Applicable	214,422	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
13170	0.751055	76,519	EXR-JFREE1eZDUKB-AN	sample_FHS_3170_No_CVD_BC_07_fastq	EXR-JFREE13170-BS	total cell-free biofluid RNA	Not Applicable	101,882	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
10320	0.946026	812,926	EXR-JFREE1eZDUKB-AN	sample_FHS_320_No_CVD_BC_07_fastq	EXR-JFREE10320-BS	total cell-free biofluid RNA	Not Applicable	859,306	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
13392	0.838254	243,884	EXR-JFREE1eZDUKB-AN	sample_FHS_3392_CVD_BC_02_fastq	EXR-JFREE13392-BS	total cell-free biofluid RNA	Not Applicable	290,943	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
13464	0.713995	59,670	EXR-JFREE1eZDUKB-AN	sample_FHS_3464_No_CVD_BC_03_fastq	EXR-JFREE13464-BS	total cell-free biofluid RNA	Not Applicable	83,572	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
13713	0.822151	148,945	EXR-JFREE1eZDUKB-AN	sample_FHS_3713_CVD_BC_06_fastq	EXR-JFREE13713-BS	total cell-free biofluid RNA	Not Applicable	181,165	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
14043	0.834504	177,837	EXR-JFREE1eZDUKB-AN	sample_FHS_4043_CVD_BC_04_fastq	EXR-JFREE14043-BS	total cell-free biofluid RNA	Not Applicable	213,105	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
14820	0.80196	175,885	EXR-JFREE1eZDUKB-AN	sample_FHS_4820_No_CVD_BC_13_fastq	EXR-JFREE14820-BS	total cell-free biofluid RNA	Not Applicable	219,319	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
14912	0.701753	46,449	EXR-JFREE1eZDUKB-AN	sample_FHS_4912_CVD_BC_02_fastq	EXR-JFREE14912-BS	total cell-free biofluid RNA	Not Applicable	66,190	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
10500	0.689864	90,586	EXR-JFREE1eZDUKB-AN	sample_FHS_500_No_CVD_BC_09_fastq	EXR-JFREE10500-BS	total cell-free biofluid RNA	Not Applicable	131,310	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
15237	0.822248	151,001	EXR-JFREE1eZDUKB-AN	sample_FHS_5237_CVD_BC_06_fastq	EXR-JFREE15237-BS	total cell-free biofluid RNA	Not Applicable	183,644	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10543	0.757923	91,811	EXR-JFREE1eZDUKB-AN	sample_FHS_543_CVD_BC_16_fastq	EXR-JFREE10543-BS	total cell-free biofluid RNA	Not Applicable	121,135	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	NO	Human	Entire cardiovascular system	Plasma
10590	0.842146	150,126	EXR-JFREE1eZDUKB-AN	sample_FHS_590_N0_CVD_BC_15_fastq	EXR-JFREE10590-BS	total cell-free biofluid RNA	Not Applicable	178,266	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16077	0.953763	1,378,861	EXR-JFREE1eZDUKB-AN	sample_FHS_6077_No_CVD_BC_09_fastq	EXR-JFREE16077-BS	total cell-free biofluid RNA	Not Applicable	1,445,706	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16136	0.825893	156,354	EXR-JFREE1eZDUKB-AN	sample_FHS_6136_CVD_BC_08_fastq	EXR-JFREE16136-BS	total cell-free biofluid RNA	Not Applicable	189,315	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16355	0.908901	727,498	EXR-JFREE1eZDUKB-AN	sample_FHS_6355_CVD_BC_08_fastq	EXR-JFREE16355-BS	total cell-free biofluid RNA	Not Applicable	800,415	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16684	0.825828	294,681	EXR-JFREE1eZDUKB-AN	sample_FHS_6684_CVD_BC_16_fastq	EXR-JFREE16684-BS	total cell-free biofluid RNA	Not Applicable	356,831	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16758	0.766947	185,579	EXR-JFREE1eZDUKB-AN	sample_FHS_6758_CVD_BC_14_fastq	EXR-JFREE16758-BS	total cell-free biofluid RNA	Not Applicable	241,971	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10676	0.943756	1,031,504	EXR-JFREE1eZDUKB-AN	sample_FHS_676_CVD_BC_06_fastq	EXR-JFREE10676-BS	total cell-free biofluid RNA	Not Applicable	1,092,977	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
16926	0.868186	178,532	EXR-JFREE1eZDUKB-AN	sample_FHS_6926_No_CVD_BC_03_fastq	EXR-JFREE16926-BS	total cell-free biofluid RNA	Not Applicable	205,638	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
17138	0.804494	295,317	EXR-JFREE1eZDUKB-AN	sample_FHS_7138_CVD_BC_12_fastq	EXR-JFREE17138-BS	total cell-free biofluid RNA	Not Applicable	367,084	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
17226	0.765965	466,364	EXR-JFREE1eZDUKB-AN	sample_FHS_7226_No_CVD_BC_15_fastq	EXR-JFREE17226-BS	total cell-free biofluid RNA	Not Applicable	608,858	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
10728	0.770573	128,725	EXR-JFREE1eZDUKB-AN	sample_FHS_728_CVD_BC_08_fastq	EXR-JFREE10728-BS	total cell-free biofluid RNA	Not Applicable	167,051	Cardiovascular Disorder	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma
17326	0.750356	139,705	EXR-JFREE1eZDUKB-AN	sample_FHS_7326_No_CVD_BC_11_fastq	EXR-JFREE17326-BS	total cell-free biofluid RNA	Not Applicable	186,185	Healthy Control	miRcury biofluids (Exiqon)	small RNA-Seq	YES	Human	Entire cardiovascular system	Plasma