000mouse tissue phosphorylation database2010-12-23 19:36:18https://gygi.med.harvard.edu/phosphomouse/search_results.php?search_phrase={{Symbol}}2011-05-03 22:50:504.044.0nsalathi/profile/80/nsalathiNeeraj Salathiamousegygi.med.harvard.edu851From E.L. Huttlin et al Cell, Volume 143, Issue 7, 1174-1189, 23 December 2010 Here we report the most thorough characterization of...biogpsuserspubliciframemousephosphorylationtissueFrom E.L. Huttlin et al Cell, Volume 143, Issue 7, 1174-1189, 23 December 2010 Here we report the most thorough characterization of tissue specific protein abundance and phosphorylation to date, including 12,000 proteins and 36,000 phosphorylation sites from nine mouse tissues. These data revealed distinctive and complementary protein and phosphoprotein expression profiles that support each tissue’s unique physiology. Moreover, by combining protein abundance measurements with phosphorylation observations, we could distinguish tissue-specific phosphorylation of ubiquitous proteins from phosphorylation of tissue-specific proteins. Furthermore, most phosphoproteins integrate input from multiple kinases spanning diverse signaling pathways. Overall, the ‘‘typical’’ phosphoprotein is broadly expressed yet is variably phosphorylated to tune protein function to the needs of each tissue. We now present these protein abundance and phosphorylation data as a web-based resource (http://gygi. med.harvard.edu/phosphomouse/index.php) to aid analysis of existing biological data and inspire future biological investigations.