Plugin: The Developing Cortical Neuron Transcriptome Resource

Neuronal development requires a complex choreography of transcriptional decisions to obtain a specific cellular identity. Yet the poor accessibility of individual neuronal classes has made direct measurements of gene expression difficult. We have combined nuclear-antigen targeting FACS sorting with high-resolution massively parallel RNA sequencing to provide the first resource of deep-transcriptomic data in individual sorted neuron populations. We provide here, an interactive space to explore the numerous aspects of these data including spatial and temporal usage of alternative mRNA isoforms and promoters, to a host of mRNA and long noncoding RNA (lncRNA) genes newly implicated in neuronal cell fate specification. This database enables integrated, multidimensional data mining and provides a powerful resource to generate insights into the transcriptional regulation underlying neuronal diversity in the developing cortex. DeCoN provides an exploratory interface to deep transcriptional profiling data of three clinically relevant subclasses of mouse cortical projection neurons: Callosal (CPN) Subcerebral (ScPN) Corticothalamic/Subplate neurons (CthPN) We have conducted isoform-deconvolution based differential RNA-Sequencing on sorted populations of each neuronal subclass at specified timepoints during corticogenesis: E15.5 E16.5 E18.5 P1

Species:

mouse

URL Template:

http://decon.fas.harvard.edu/pyramidal/gene/{{symbol}}

Updated:

Nov. 18, 2015

Registered:

Nov. 18, 2015