Dataset: Transcription profiling of mouse esophageal squamous cells after zinc-deficient or zinc-sufficient diet

Weanling littermates Cox-2-/- and Cox-2+/+ (WT) mice were fed a zinc-deficient (ZD) or zinc-sufficient (ZS) diet to form 4 groups, namely, ZD:Cox-2-/- (n=4), ZD:WT (n=4), ZS:Cox-2-/- (n=4) and ZS:WT (n=4). After 8 weeks, ZD mouse forestomach evidenced increased cell proliferation as demonstrated by increased proliferating cell nuclear antigen [PCNA] labeling. The mice were sacrificed and forestomachs isolated. Forestomach epithelia were prepared by using a blade to strip off the submucosal and muscularis layers under the guidance of a dissecting microscope. We performed microarray analysis on individual RNA samples prepared from 4 mice from each of the following 4 groups: ZS:Cox-2-/-, ZS:WT, ZD:Cox-2-/- and ZD:WT mice (n=4/group), using GeneChipﾮ Mouse Genome 430 2.0 Array (Affymetrix, Santa Clara, CA). All RNA samples displayed intact ribosomal RNA 18S and 28S bands as determined by the Agilent 2100 Bioanalyzer. Five micrograms of total RNA was reverse transcribed into cDNA followed by in vitro transcription and labeling to produce biotin-labeled cRNA using IVT Labeling Kit. The labeled cRNA was hybridized to the array, followed staining with streptavidin-phycoerythrin. The distribution of fluorescent material on the oligonucleotide probes sets was obtained using GeneArray Scanner

Species:

mouse

Samples:

16

Source:

E-TABM-778

Updated:

Dec.12, 2014

Registered:

Nov.21, 2014