{"platform": 6, "owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"UOB-2_sr2_DMSO24h_01": {"Time": 24, "Compound": "control"}}, {"UOB-2_sr2_DMSO24h_01": {"Time": 24, "Compound": "control"}}, {"UOB-2_sr2_DMSO24h_01": {"Time": 24, "Compound": "control"}}, {"UOB-2_sr2_DMSO48h_01": {"Time": 48, "Compound": "control"}}, {"UOB-2_sr2_DMSO48h_01": {"Time": 48, "Compound": "control"}}, {"UOB-2_sr2_DMSO48h_01": {"Time": 48, "Compound": "control"}}, {"UOB-2_sr2_DMSO72h_01": {"Time": 72, "Compound": "control"}}, {"UOB-2_sr2_DMSO72h_01": {"Time": 72, "Compound": "control"}}, {"UOB-2_sr2_DMSO72h_01": {"Time": 72, "Compound": "control"}}, {"UOB-2_sr2_LY24h_01": {"Time": 24, "Compound": "LY294002"}}, {"UOB-2_sr2_LY24h_01": {"Time": 24, "Compound": "LY294002"}}, {"UOB-2_sr2_LY24h_01": {"Time": 24, "Compound": "LY294002"}}, {"UOB-2_sr2_LY48h_01": {"Time": 48, "Compound": "LY294002"}}, {"UOB-2_sr2_LY48h_01": {"Time": 48, "Compound": "LY294002"}}, {"UOB-2_sr2_LY48h_01": {"Time": 48, "Compound": "LY294002"}}, {"UOB-2_sr2_LY72h_01": {"Time": 72, "Compound": "LY294002"}}, {"UOB-2_sr2_LY72h_01": {"Time": 72, "Compound": "LY294002"}}, {"UOB-2_sr2_LY72h_01": {"Time": 72, "Compound": "LY294002"}}], "id": 8200, "ownerprofile_id": "arrayexpress_sid", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-674", "summary_wrapped": "[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th...", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 18, "tags": ["cell", "column", "dish", "glutamine", "line", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-mouse-embyronic-stem-3", "geo_id_plat": "E-TABM-674_A-AFFY-45", "name": "Transcription profiling of mouse embyronic stem cells cultured with PI3-K signalling inhibitor LY294002 for different lengths of time to identify PI3K-target genes", "created": "Nov.21, 2014", "summary": "[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, ', {u'a': {u'href': u'http://www.fungenes.org', u'target': u'_blank', u'$': u'http://www.fungenes.org'}}, u').The experiment was conducted at the Unyversity of Bath, Bath, UK. Aim is to identify genes regulated by PI3K-dependent signaling in undifferentiated ES cells. Materials and methods: E14tg2a cell line was used. Cells were cultured in Knock-out DMEM supplemented with 15% (v/v) Knock-out serum replacement, 1000U/ml LIF,  2mM glutamine, 50 ?M 2-mercaptothanol and 1x NEAA (as described in Handbook of work methods, 4.3.1.1). Cells were plated at 5x105 per 10cm gelatin-coated TC dish for 24h. ES cells were then starved for 24h in media lacking LIF and pre-incubated for 30 minutes with 5 <B5>M LY294002 (the concentration of this PI3K inhibitor that we have shown to perturb self-renewal in the presence of LIF and which knocks down PI3K signals) or DMSO alone. Cells were then stimulated with 103U/ml mLIF (Chemicon) for 2h (which gave maximal activation of STAT3), 24h, 48h or 72h.</p>\\\\n<h3>Relationships between samples</h3>\\\\n <p  ALIGN=\"JUSTIFY\">Samples were either maintained in LIF or LIF plus LY294002 (PI3K inhibitor) for the required treatment times.</p>\\\\n<h3>Treatments</h3>\\\\n <p  ALIGN=\"JUSTIFY\">Treatment times with PI3K inhibitor LY294002 at 5 <B5>M <96> 2h (n=5), 24h (n=3), 48h (n=3), 72h (n=3). DMSO was used as vehicle alone treatment control.</p>\\\\n<h3>Culture conditions</h3>\\\\n <p  ALIGN=\"JUSTIFY\">Gelatin-coated TC dishes (Nunc)</p>\\\\n<h3>RNA isolation method</h3>\\\\n <p  ALIGN=\"JUSTIFY\">RNA Easy with on-column DNase treatment</p>\\\\n<h3>Experimental description</h3>\\\\n <p  ALIGN=\"JUSTIFY\">This information is included above. Briefly, E14tg2a ES cells were either maintained in LIF or LIF plus LY294002 (5 <B5>M) for 2, 24, 48 or 72 hours, after which RNA was extracted from each sample and sent for expression profiling. During this time-course, only 72h time points plus LY294002 showed phenotypic evidence of differentiation. </p>\\\\n']", "geo_gse_id": "E-TABM-674", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-674/samples/"}