{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"UOB-1_sr1_DMSO_01": {"compound": "DMSO control", "dose": "    "}}, {"UOB-1_sr1_DMSO_01": {"compound": "DMSO control", "dose": "    "}}, {"UOB-1_sr1_DMSO_01": {"compound": "DMSO control", "dose": "    "}}, {"UOB-1_sr1_DMSO_01": {"compound": "DMSO control", "dose": "    "}}, {"UOB-1_sr1_DMSO_01": {"compound": "DMSO control", "dose": "    "}}, {"UOB-1_sr1_LY_01": {"compound": "LY294002", "dose": "5"}}, {"UOB-1_sr1_LY_01": {"compound": "LY294002", "dose": "5"}}, {"UOB-1_sr1_LY_01": {"compound": "LY294002", "dose": "5"}}, {"UOB-1_sr1_LY_01": {"compound": "LY294002", "dose": "5"}}, {"UOB-1_sr1_LY_01": {"compound": "LY294002", "dose": "5"}}], "id": 8199, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th...", "pubmed_id": 19350676, "geo_gse_id": "E-TABM-673", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 10, "tags": ["serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-by-array-of-mouse-embryo-6", "geo_id_plat": "E-TABM-673_A-AFFY-45", "name": "Transcription profiling by array of mouse embryonic stem cells cultured with LY294002", "created": "Nov.21, 2014", "summary": "[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, ', {u'a': {u'href': u'http://www.fungenes.org', u'target': u'_blank', u'$': u'http://www.fungenes.org'}}, u').The experiment was conducted at the Unyversity of Bath, Bath, UK. Aim of the experiment is the identification of genes regulated by PI3K-dependent signaling in undifferentiated ES cells. Materials and methods: To identify target genes of PI3-K signalling, the PI3-K signalling inhibitor LY294002 was used at a concentration which was shown to perturb self-renewal in the presence of LIF and which knocks down PI3K signals. CGR8 cells cultivated in serum-free conditions in the presence of LIF were starved for 24h in media lacking LIF and then pre-inubated for 30 minutes with 5 mM LY294002 in DMSO or with DMSO alone. Cells were then stimulated with LIF for 2h, prior to preparation of RNA samples. Relationships between samples: Samples were either maintained in LIF or LIF plus LY294002 (PI3K inhibitor) for the required treatment times. Treatments: Treatment times with PI3K inhibitor LY294002 at 5 uM 2h (n=5), 3h (n=5).']", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-673", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-673/samples/"}