ArrayExpress Uploader081930.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.0control0.0control0.0control0.0control0.0control0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.42Leukemia Inhibitory Factor0.0control0.0control0.0control0.0control0.0control48.42Leukemia Inhibitory Factor48.42Leukemia Inhibitory Factor48.42Leukemia Inhibitory Factor48.42Leukemia Inhibitory Factor48.42Leukemia Inhibitory Factor48.0Leukemia Inhibitory Factor48.0Leukemia Inhibitory Factor48.0Leukemia Inhibitory Factor48.0Leukemia Inhibitory Factor48.0Leukemia Inhibitory Factorarrayexpress_sid6[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th...19203379E-TABM-667/profile/8773/arrayexpressuploader230cellcolumninterleukininterleukin-6leukemialineserumDec.12, 2014Falsetranscription-profiling-of-mouse-embryonic-stem-15E-TABM-667_A-AFFY-45Transcription profiling of mouse embryonic stem cell line CGR8 treated with Leukemia Inhibitory Factor (LIF) to identify LIF-dependent genes and early differentiation markersNov.21, 2014[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, ', {u'a': {u'href': u'http://www.fungenes.org', u'target': u'_blank', u'$': u'http://www.fungenes.org'}}, u'). The experiment was conducted at the University Bordeaux 2 (CNRS UMR 5164). The purpose of the experiment was to identify the LIF-dependent genes and early differentiation markers in mouse ES cells. Leukemia Inhibitory Factor (LIF) is a member of the Interleukin-6 (IL-6) family of cytokines, which displays pleiotropic functions, depending on both cell maturity and cell type. LIF maintains ES cell pluripotency and is a critical cell survival factor in myocytes. Materials and methods: CGR8 cell line was used.6 cell conditions tested: + LIF, reinduced 25 min with serum + LIF reinduced 25 min with serum and LIF 500pM - LIF for 24h, reinduced 25 min with serum - LIF for 24h, reinduced 25 min with serum and LIF 500pM- LIF for 48h, reinduced 25 min with serum - LIF for 48h, reinduced 25 min with serum and LIF 500pM. Relationships between samples. 5 independent preparations of mRNA have been done on five different CGR8 passages. Culture conditions: Monolayer adherent cells. RNA isolation method: Qiagen column']http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-667mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-667/samples/