Dataset: Transcription profiling of mouse embryonic stem cells to identify genes regulated by STAT3 and NANOG, known differentiation inhibitors

[u'This experiment is part of the FunGenES project (FunGenES - Functional Genomics in Embryonic Stem Cells partially funded by the 6th Framework Programme of the European Union, ', {u'a': {u'href': u'http://www.fungenes.org', u'target': u'_blank', u'$': u'http://www.fungenes.org'}}, u'). The experiment was conducted at the Inst. National de la Sant\xe9 et de la Recherche M\xe9dicale - INS (INSERM U371, Bron, France) The goal of the experiment is to identify genes regulated by STAT3 and NANOG, which are known inhibitors of mouse ES cell differentiation.(I) E14-Cre-CTL cells deprived of LIF for 24 hours (n = 5); (II) E14-Cre-Tax cells as in (I) and restimulated with 100 nM tamoxifen for 2 hours (n = 5); (III) E14-STATA3-CTL cells deprived of LIF and 4\u2019OHT for 24 hours (n = 4); (IV) E14-STAT3-LIF cells treated as in (III) and re-stimulated with 10,000 U/ml LIF for 1 hour to reactivate endogenous STAT3 (n = 5); (V) E14-STAT3-Tax cells as in (III) and restimulated with 100 nM 4\u2019OHT for 2 hours to activate STAT3-ERT2 (n = 6);']

Species:

mouse

Samples:

25

Source:

E-TABM-562

Updated:

Dec.12, 2014

Registered:

Nov.21, 2014