ArrayExpress Uploaderarrayexpress_sidhumanFGF stimulation3FGF stimulation4FGF stimulation6FGF stimulation8GM-CSF stimulation1GM-CSF stimulation2GM-CSF stimulation3GM-CSF stimulation4GM-CSF stimulation6GM-CSF stimulation8uninduced0SDF stimulation1SDF stimulation2SDF stimulation3SDF stimulation4SDF stimulation6SDF stimulation8uninduced0HGF stimulation1HGF stimulation2HGF stimulation3HGF stimulation4HGF stimulation6HGF stimulation8FGF stimulation1FGF stimulation2523904Cellular decisions leading to a sustained cellular migration constitute a critical parameter during wound healing and metastasis....E-TABM-440/profile/8773/arrayexpressuploader226cellskinDec.12, 2014FalseE-TABM-440_A-AFFY-44transcription-profiling-of-human-keratinocytes-aftTranscription profiling of human keratinocytes after stimulation with recombinant human cytokines to study the events controlling cell migration)Sep.22, 2014Cellular decisions leading to a sustained cellular migration constitute a critical parameter during wound healing and metastasis. Cellular decision processes for migration involve signaling pathways as well as gene expression regulation that have been not yet studied in an integrated way. Here, heterologous cocultures combining primary human keratinocytes and genetically defined murine fibroblasts served as a model for wound healing of human skin to gain a systemic view of the underlying principles of cellular decision making towards migration. By inverse modeling of time series of gene expression data followed by experimental validation we show that (I) pulse-like activation of the proto-oncogene receptor Met by its ligand HGF triggers a responsive system state, (II) permanent, time sequential activation of the EGF-receptor is required to initiate and sustain migration, and (III), context information for enhancing, delaying or stopping migration is provided via the activity of the PKA-signaling pathway. Our study reveals a complex orchestration of events controlling cell migration.http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-440http://www.ebi.ac.uk/arrayexpress/experiments/E-TABM-440/samples/