BioGPS
  • Home
  • Help
  • Plugins
  • Datasets
  • Sign Up
  • Login
Examples: Gene Symbol(s), Gene Ontology, Splicing plugins, Melanoma datasets
advanced
Home › Dataset Library › Transcription profiling by array of human MCF7 cells expressing FOXP3

Dataset: Transcription profiling by array of human MCF7 cells expressing FOXP3

As we clarified before, the FOXP3 gene is an X-linked tumor suppressor gene in mammary carcinoma in both human and mouse. We also...

Registered by ArrayExpress Uploader
View Dataset

As we clarified before, the FOXP3 gene is an X-linked tumor suppressor gene in mammary carcinoma in both human and mouse. We also clarified that the ERBB2 and SKP2 oncogenes were transcriptionally under control of the FOXP3 gene product in mammary epithelial cells. In order to further clarify the FOXP3 down stream target genes in human breast cancer cells, we planed to conduct a microarray analysis of FOXP3-induced gene expression profiling. MCF7, a human breast cancer cell line, with FOXP3-tet-off system was cultured with and without Doxycyclin for 2 days. In the MCF7 cell cultured without Dox, the FOXP3 transcript was significantly induced as compared to the MCF7 cultured with Dox. Total RNA from MCF7 with and wihtout Dox were extracted by Qiagen's RNeasy column and they were applied to Affymetrix Human U133 2.0 array according to the manufacture's protocol. We clarified as yet unknown FOXP3 target genes in human epithelial cells, e.g., the p21 gene, by this analysis.

Species:
human

Samples:
10

Source:
E-MTAB-73

PubMed:
19276356

Updated:
Dec.12, 2014

Registered:
Sep.22, 2014


Factors: (via ArrayExpress)
Sample phenotype
FOXP3plus1 expression of FOXP3
FOXP3plus1 expression of FOXP3
FOXP3plus1 expression of FOXP3
FOXP3plus1 expression of FOXP3
FOXP3plus1 expression of FOXP3
FOXP3minus1 doxycycline-mediated repression of FOXP3
FOXP3minus1 doxycycline-mediated repression of FOXP3
FOXP3minus1 doxycycline-mediated repression of FOXP3
FOXP3minus1 doxycycline-mediated repression of FOXP3
FOXP3minus1 doxycycline-mediated repression of FOXP3

Tags

  • breast
  • breast cancer
  • cancer
  • carcinoma
  • cell
  • column
  • line
  • mammary carcinoma

Other Formats

JSON    XML
  • About
  • Blog
  • Help
  • FAQ
  • Downloads
  • API
  • iPhone App
  • Email updates
© 2022 The Scripps Research Institute. All rights reserved. (ver 94eefe6 )
  • Terms of Use