ArrayExpress Uploader0human2D monolayer without EGF and insulin4-hydroxytamoxifen21002D monolayer without EGF and insulin4-hydroxytamoxifen481002D monolayer without EGF and insulinnone48 2D monolayer with EGF and insulin4-hydroxytamoxifen21002D monolayer with EGF and insulin4-hydroxytamoxifen481002D monolayer with EGF and insulinnone48 3D Matrigel culture4-hydroxytamoxifen21003D Matrigel culture4-hydroxytamoxifen481003D Matrigel culturenone48 5199arrayexpress_sid4MCF10A MycER cells were grown either on 2D monolayer culture in the presence of complete growth medium, in the absence of EGF and insulin...E-MEXP-765/profile/8773/arrayexpressuploader49insulinDec.12, 2014Falsetranscription-profiling-by-array-of-human-mammar-3E-MEXP-765_A-AFFY-44Transcription profiling by array of human mammary epithelial cells grown in 2D monolayers with or without EGF and insulin, or 3D Matrigel culture, after treatment with 4-hydroxytamoxifenSep.22, 2014MCF10A MycER cells were grown either on 2D monolayer culture in the presence of complete growth medium, in the absence of EGF and insulin or in 3D Matrigel culture for 20 days to form acinar structures. MycER was activated with 4-hydroxytamoxifen (4-OHT) in all samples for 2 or 48 h. Control cells were treated with ethanol. The aim of the study was to explore the effect of different growth conditions on gene expression changes in response to c-Myc activation.http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-765http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-765/samples/