Dataset: Transcription profiling by array of human peripheral blood lymphocytes or haemopoietic stem cells at the time of transduction with a gammaretroviral vector

Expression profile comparison of peripheral blood lymphocytes (PBL) or haemopoietic stem cells (HSC) at the time of transduction with a gammaretroviral vector. The analysis is performed in the context of two different gene therapy clinical trials (Recchia et al, 2006; Aiuti et al, 2007) based on autologous infusion of human T cells or CD34+ haemopoietic progenitor cells gene-corrected with an moloney leukemia virus-derived vector (MLV-vector). T cells and CD34+ cells were isolated from patients and pre-stimulated in vitro with cytokines prior to transduction with the vector and re-infusion in patient. The expression profiles of T cells and CD34+ cells were analysed by microarray just after cytokine stimulation, providing information on the transcriptional activity of these two target cells at the time of transduction.

Species:

human

Samples:

4

Source:

E-MEXP-2983

Updated:

Dec.12, 2014

Registered:

Jun.19, 2014