{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"E1_B3": {"DOSE": 0, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E1_B3": {"DOSE": 0, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E1_B3": {"DOSE": 0, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E1_V1": {"DOSE": 0, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E1_V1": {"DOSE": 0, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E1_V1": {"DOSE": 0, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_B1": {"DOSE": 100, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_B1": {"DOSE": 100, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_B1": {"DOSE": 100, "GENOTYPE": "Basp", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_V1": {"DOSE": 100, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_V1": {"DOSE": 100, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}, {"E2_V1": {"DOSE": 100, "GENOTYPE": "Vector control", "COMPOUND": "phorbol 12-myristate 13-acetate"}}], "id": 5156, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "We have analysed the dynamic between WT1 and BASP1 in the regulation of gene expression in myelogenous leukaemia K562 cells. Our analysis...", "geo_gse_id": "E-MEXP-2573", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 12, "tags": ["blood cell", "cell", "wt1"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "transcription-profiling-of-human-myelogenous-leuke", "geo_id_plat": "E-MEXP-2573_A-AFFY-44", "name": "Transcription profiling of human myelogenous leukemia K562 cells expressing BASP1", "created": "Sep.22, 2014", "summary": "We have analysed the dynamic between WT1 and BASP1 in the regulation of gene expression in myelogenous leukaemia K562 cells. Our analysis reveals that BASP1 is a significant regulator of WT1 that is recruited to WT1-binding sites and suppresses WT1-mediated transcriptional activation at several WT1 target genes. We found that WT1 and BASP1 can divert the differentiation program of K562 cells to a non-blood cell type following induction by the phorbol ester PMA. Cell lines were generated expressing the BASP1 gene from the vector pcDNA3. Array analysis was performed on 4 conditions: cells expressing BASP1 and controls, in the absence and presence of Phorbol 12-myristate 13-acetate (PMA).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2573", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2573/samples/"}