ArrayExpress Uploader05151cortisol100cortisol100cortisol100cortisol200cortisol200cortisol200none none none cortisol100 and 200 oscillatingcortisol100 and 200 oscillatingcortisol100 and 200 oscillatingarrayexpress_sid4Glucocorticoids act through the glucocorticoid receptor (GR), a ligand activated transcription factor, to modulate gene expression...21267416E-MEXP-2447/profile/8773/arrayexpressuploader212adrenal glandcellglandDec.12, 2014Falsetranscription-profiling-by-array-of-human-hela-c-2E-MEXP-2447_A-AFFY-44Transcription profiling by array of human HeLa cells treated with pulsatile cortisolSep.22, 2014Glucocorticoids act through the glucocorticoid receptor (GR), a ligand activated transcription factor, to modulate gene expression profiles in target cells and tissues. The principal physiological glucocorticoid in humans, cortisol, is released in a pulsatile fashion from the adrenal gland, which results in oscillations in blood concentrations, with a period of 1 - 2 hours. The biological consequence of fluctuating cortisol concentrations has not been explored. To identify the role of cortisol concentration oscillations for target cell response a flow-through cell culture system was developed. Transcription profiling was performed in the absence and presence of cortisol. Cells were exposed to constant cortisol levels of 100ng/ml or 200 ng/ml or oscillating cortisol concentrations.http://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2447humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-MEXP-2447/samples/